A comparison of the patterns of migration and the destinations of homotopically transplanted neonatal subventricular zone cells and heterotopically transplanted telencephalic ventricular zone cells.
A comparison of the patterns of migration and the destinations of homotopically transplanted neonatal subventricular zone cells and heterotopically transplanted telencephalic ventricular zone cells.
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同位移植的新生儿室下区细胞和异位移植的端脑室区细胞的迁移模式和目的地的比较。
DOI：
10.1006/dbio.1996.0040
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Luskin,MB
中科院分区：
文献类型：
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作者：
Zigova,T;Betarbet,R;Soteres,BJ;Brock,S;Bakay,RA;Luskin,MB
室下区（SVZa）前部产生的细胞沿着缺乏放射状胶质纤维的界限分明的通路迁移到嗅球，在那里它们分化成颗粒细胞层或肾小球层的中间神经元（Luskin，1993，Neuron11，173）。为了分析这种高度定向迁移的机制，我们将未操作的 SVZa 衍生细胞的迁移行为与同位移植的 SVZa 细胞和异位移植的端脑室区 (VZ) 细胞的迁移行为进行了比较，这些细胞通常与放射状胶质纤维相关迁移。为了在移植前鉴定 SVZa 祖细胞的表型，我们使用细胞类型特异性标记物在体外对其进行了表征。培养 1 天后，几乎所有 SVZa 细胞都被 TuJ1（一种神经元特异性标记物）染色；根据 GFAP 免疫反应性的存在判断，只有偶尔的细胞表现出神经胶质表型。这表明 SVZa 细胞表达神经元表型。为了揭示同位移植的新生 SVZa 细胞在宿主大脑中的时空分布，将出生后第 0 天 (P0)–P2 动物的分离 SVZa 细胞用亲脂性染料 PKH26 或细胞增殖标记物 BrdU 标记，并植入同龄宿主动物的 SVZa 中。移植后第一周内，整个通路中出现大量标记细胞。在接下来的 2 周内，标记的细胞迁移到嗅球的上层细胞层并开始分化，并且在 4 周内，移植的细胞以与未操作的 SVZa 衍生细胞相同的比例到达嗅球的颗粒细胞和肾小球层中的最终位置。在前往嗅球的途中，同位移植的细胞从未偏离迁移路径。相比之下，来自胚胎端脑的异位移植VZ细胞虽然确实分化，但并未发生迁移。这些结果表明，同位移植的 SVZa 衍生细胞采用的迁移模式与 SVZa 衍生神经元通常使用的迁移模式没有区别，并且 VZ 细胞无法破译同一组引导信号。
The cells arising in the anterior part of the subventricular zone (SVZa) migrate along a well-demarcated pathway which lacks radial glial fibers to the olfactory bulb where they differentiate into interneurons of the granule cell layer or glomerular layer (Luskin, 1993,Neuron11, 173). To analyze the mechanisms underlying this highly directed migration, we have compared the migratory behavior of unmanipulated SVZa-derived cells to that of homotopically transplanted SVZa cells and of heterotopically transplanted telencephalic ventricular zone (VZ) cells that ordinarily migrate in association with radial glial fibers. To identify the phenotype of the SVZa progenitor cells prior to their transplantation, we characterized themin vitrousing cell type-specific markers. After 1 day in culture nearly all the SVZa cells were stained with TuJ1, a neuron-specific marker; only an occasional cell exhibited a glial phenotype as judged by the presence of GFAP-immunoreactivity. This indicates that SVZa cells express a neuronal phenotype. To reveal the spatiotemporal distribution of homotopically transplanted neonatal SVZa cells in a host brain, dissociated SVZa cells from Postnatal Day 0 (P0)–P2 animals were labeled with the lipophilic dye PKH26 or the cell proliferation marker BrdU and implanted into the SVZa of host animals of the same age. Within the first week after transplantation there were vast numbers of labeled cells throughout the pathway. Over the next 2 weeks the labeled cells migrated into the overlying cellular layer of the olfactory bulb and began to differentiate, and within 4 weeks the transplanted cells had reached their final positions in the granule cell and glomerular layers of the olfactory bulb in the same proportions as for unmanipulated SVZa-derived cells. While en route to the olfactory bulb the homotopically transplanted cells never strayed from the migratory pathway. In contrast, heterotopically transplanted VZ cells from the embryonic telencephalon did not undergo migration although they did differentiate. These results demonstrate that the homotopically transplanted SVZa-derived cells adopt a mode of migration indistinguishable from that ordinarily utilized by SVZa-derived neurons and that the VZ cells are unable to decipher the same set of guidance cues.