Determinants of spontaneous activity in networks of cultured hippocampus
Determinants of spontaneous activity in networks of cultured hippocampus
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DOI：
10.1016/j.brainres.2008.06.022
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发表时间：
2008-10-15
期刊：
影响因子：
2.9
通讯作者：
Segal, Menahem
中科院分区：
文献类型：
--
作者：
Cohen, Eyal;Ivenshitz, Miriam;Segal, Menahem
大脑产生广泛的自发网络活动模式，即使在没有外部传入。虽然这些复杂活动的认知相关性正在被解开，但大脑中不同内在网络活动模式的产生、同步和传播的规则仍然是个谜。利用在分离培养物中生长的海马神经元，我们能够研究这些规则。网络活动出现在3-7天的体外（DIV）独立于正在进行的兴奋性或抑制性突触活动。网络活动在接下来的几周内成熟，当它对慢性药物治疗变得敏感时。网络的大小决定了它的属性，因此密集网络的同步活动率高于稀疏网络，稀疏网络的同步性更高，但节奏更低。大型网络不能通过激活单个神经元来触发。另一方面，小网络不会自发地爆发，但可以通过刺激单个神经元来释放网络爆发。因此，连接的强度与自发活动和同步性呈负相关。在确认的“领导者”神经元的情况下，同步爆发网络活动似乎是由至少几个局部阈下突触事件触发的。我们的结论是，在文化中的神经元网络可以产生自发的同步活动，并作为一个可行的模型系统，用于分析的规则，在大脑中的网络活动，（c）2008年爱思唯尔B. V.保留所有权利。
The brain generates extensive spontaneous network activity patterns, even in the absence of extrinsic afferents. While the cognitive correlates of these complex activities are being unraveled, the rules that govern the generation, synchronization and spread of different patterns of intrinsic network activity in the brain are still enigmatic. Using hippocampal neurons grown in dissociated cultures, we are able to study these rules. Network activity emerges at 3-7 days in-vitro (DIV) independent of either ongoing excitatory or inhibitory synaptic activity. Network activity matures over the following several weeks in culture, when it becomes sensitive to chronic drug treatment. The size of the network determines its properties, such that dense networks have higher rates of less synchronized activity than that of sparse networks, which are more synchronized but rhythm at lower rates. Large networks cannot be triggered to fire by activating a single neuron. Small networks, on the other hand, do not burst spontaneously, but can be made to discharge a network burst by stimulating a single neuron. Thus, the strength of connectivity is inversely correlated with spontaneous activity and synchronicity. In the absence of confirmed 'leader' neurons, synchronous bursting network activity appears to be triggered by at least several local subthreshold synaptic events. We conclude that networks of neurons in culture can produce spontaneous synchronized activity and serve as a viable model system for the analysis of the rules that govern network activity in the brain, (c) 2008 Elsevier B.V. All rights reserved.