microRNA expression in hepatitis B virus infected primary treeshrew hepatocytes and the independence of intracellular miR-122 level for de novo HBV infection in culture.
microRNA expression in hepatitis B virus infected primary treeshrew hepatocytes and the independence of intracellular miR-122 level for de novo HBV infection in culture.
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DOI：
10.1016/j.virol.2013.10.016
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发表时间：
2014-01
期刊：
影响因子：
3.7
通讯作者：
Guangwei Xu;Zhenchao Gao;Wenhui He;Yanting Ma;Xiaofeng Feng;Tao Cai;F. Lu;Li Liu;Wenhui Li
中科院分区：
文献类型：
--
作者：
Guangwei Xu;Zhenchao Gao;Wenhui He;Yanting Ma;Xiaofeng Feng;Tao Cai;F. Lu;Li Liu;Wenhui Li
B型肝炎病毒（HBV）在肝细胞中的感染是由多种细胞因子控制的，但其与肝脏microRNA的关系尚不清楚。在这项研究中，一个miRNA数据库，包含168个独特的成熟的miRNA成员从灵长类动物，北方树鼩（树鼩），是唯一的物种易感HBV感染以外的人和黑猩猩的原代肝细胞，建立。肝脏主要microRNA，miR-122的相对水平在HBV感染原代树鼩肝细胞（PTH）后显著增加。然而，将miR-122或其拮抗剂anti-miR-122引入PTH或HepG 2-NTCP（即具有新鉴定的受体牛磺胆酸钠共转运多肽（NTCP）的HepG 2细胞）中并不改变这些细胞上的病毒感染。这些数据表明HBV对培养肝细胞的重新感染不依赖于靶细胞内miR-122的表达水平。
Infection of Hepatitis B virus (HBV) in hepatocytes has been known to be controlled by multiple cellular factors, while the relationship of the infection and liver microRNAs remains obscure. In this study, a miRNA database, containing 168 unique mature miRNA members from primary hepatocytes of a primate-like animal, northern treeshrew (Tupaia belangeri) that is the only species susceptible for HBV infection other than human and chimpanzee, was established. The relative level of a liver predominant microRNA, miR-122, was markedly increased upon HBV infection of the primary tupaia hepatocyte (PTH). However, introducing neither miR-122 nor its antagonist anti-miR-122 into PTHs, or, HepG2–NTCP that is HepG2 cells with the newly identified receptor sodium taurocholate cotransporting polypeptide (NTCP) did not alter the viral infection on these cells. These data suggest thatde novoHBV infection of cultured hepatocytes does not depend on the expression level of intracellular miR-122 of the target cells.