Optical monitoring and treatment of potentially lethal wound infections in vivo
Optical monitoring and treatment of potentially lethal wound infections in vivo
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DOI：
10.1086/375244
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发表时间：
2003-06-01
影响因子：
6.4
通讯作者：
Hasan, T
中科院分区：
文献类型：
--
作者：
Hamblin, MR;Zahra, T;Hasan, T
我们报告使用光学技术监测和治疗铜绿假单胞菌伤口感染的小鼠。用含有细菌lux基因操纵子的质粒转导的生物发光细菌允许通过使用敏感的电荷耦合器件照相机对切除的小鼠伤口中的感染进行成像。通过使用聚阳离子光敏剂缀合物进行光动力疗法（PDT）靶向细菌，所述聚阳离子光敏剂缀合物被设计成穿透革兰氏阴性细胞壁，并且局部施加到伤口，然后进行红光照射。如通过图像分析所测量的，在用缀合物和光处理的伤口中存在快速的光剂量依赖性发光损失，在未处理的伤口、仅用光处理的伤口或仅用缀合物处理的伤口中未观察到这种损失。铜绿假单胞菌在我们的小鼠模型中具有侵袭性，所有3组对照小鼠均在5天内死亡;相比之下，90%的PDT治疗小鼠存活。PDT治疗的伤口愈合速度明显快于银硝酸盐治疗的伤口，这不是由于银抑制愈合或PDT刺激愈合。
We report on the use of optical techniques to monitor and treat Pseudomonas aeruginosa wound infections in mice. Bioluminescent bacteria transduced with a plasmid containing a bacterial lux gene operon allow the infection in excisional mouse wounds to be imaged by use of a sensitive charge-coupled device camera. Photodynamic therapy (PDT) targeted bacteria, by use of a polycationic photosensitizer conjugate, which is designed to penetrate the gram-negative cell wall and was topically applied to the wound and was followed by red-light illumination. There was a rapid light dose-dependent loss of luminescence, as measured by image analysis, in the wounds treated with conjugate and light, a loss that was not seen in untreated wounds, wounds treated with light alone, or wounds treated with conjugate alone. P. aeruginosa was invasive in our mouse model, and all 3 groups of control mice died within 5 days; in contrast, 90% of PDT-treated mice survived. PDT-treated wounds healed significantly faster than did silver nitrate-treated wounds, and this was not due to either inhibition of healing by silver nitrate or stimulation of healing by PDT.