DARPP-32 DEVELOPMENT IN THE CAUDATE-NUCLEUS IS INDEPENDENT OF AFFERENT INPUT FROM THE SUBSTANTIA-NIGRA
DARPP-32 DEVELOPMENT IN THE CAUDATE-NUCLEUS IS INDEPENDENT OF AFFERENT INPUT FROM THE SUBSTANTIA-NIGRA
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DOI：
10.1016/0165-3806(90)90148-r
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发表时间：
1990-07-01
期刊：
影响因子：
--
通讯作者：
GREENGARD, P
中科院分区：
文献类型：
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作者：
EHRLICH, ME;ROSEN, NL;GREENGARD, P
DARPP-32是一种多巴胺和腺苷调节的神经元磷酸蛋白，分子量32 kDa，是哺乳动物尾壳核中型棘神经元的表型标志物。在本研究中，我们研究了DARPP-32蛋白和mRNA的个体发育，并将其与酪氨酸羟基酶和突触-囊泡磷蛋白突触蛋白I的个体发育进行了比较。在体内，每毫克总蛋白中的DARPP-32蛋白的含量在出生后前三周一直在增加，然后在成年后下降到平台期。MRNA水平与蛋白质水平密切相关，除了它的上升先于蛋白质的上升。酪氨酸羟基酶水平在出生后的前4周一直在上升，突触素I的水平在同一时期稳步上升。含有尾壳核细胞的原代重组培养物表达DARPP-32，其时间过程与体内看到的相似。与来自吻侧中脑被盖的多巴胺能神经元共培养，其表达水平没有改变。因此，出生后尾壳核中DARPP-32水平的增加似乎与黑质的跨突触或终末器官的影响无关。
DARPP-32, a dopamine- and adenosine 3'':5''-monophosphate regulated neuronal phosphoprotein, Mr 32 kDa, is a phenotypic marker of the medium-size spiny neurons of the mammalian caudate-putamen. In the present study, we examined the ontogeny of DARPP-32 protein and mRNA, and compared it to the ontogeny of tyrosine hydroxylase and synapsin I, a synaptic-vesicle phosphoprotein. In vivo, the amount of DARPP-32 protein per mg total protein increased throughout the first three postnatal weeks, and then declined to plateau at adult levels. The mRNA level closely paralleled the protein, except that its rise preceded that of the protein. Tyrosine hydroxylase levels rose throughout the first 4 postnatal weeks, and synapsin I levels rose steadily during the same period. Primary reaggregate cultures containing cells from the caudate-putamen expressed DARPP-32 with a time course similar to that seen in vivo. The level of expression was not altered by coculturing with dopaminergic neurons from the rostral mesencephalic tegmentum. Thus, the postnatal increase in DARPP-32 levels in the caudate-putamen appears to be independent of transsynaptic or end-organ influences from the substantia nigra.