Polyunsaturated fatty acids-enriched lipid from reduced sugar alcohol mannitol by marine yeast Rhodosporidiobolus fluvialis Y2
Polyunsaturated fatty acids-enriched lipid from reduced sugar alcohol mannitol by marine yeast Rhodosporidiobolus fluvialis Y2
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DOI：
10.1016/j.bbrc.2020.04.032
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发表时间：
2020-06-11
影响因子：
3.1
通讯作者：
Hashimoto, Wataru
中科院分区：
文献类型：
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作者：
Nakata, Shota;Hio, Mamoru;Hashimoto, Wataru
大型褐藻是一种很有前途的海洋生物质，可用于生产生物乙醇和生物柴油。在这里，我们调查海洋产油酵母同化褐藻中发现的主要碳水化合物的生化过程。简言之，从海水中分离酵母Rhodosporidiobolus fluvialis菌株Y2，并在含有还原糖醇甘露醇作为唯一碳源的基本培养基中生长，盐度与海水相当。限制氮的条件用于促进脂质合成。R.在盐度存在和不存在的情况下，河生酵母Y2从甘露醇中分别获得每干细胞重55.1%（w/w）和39.1%（w/w）的脂质。此外，甘露醇，作为糖源，导致多不饱和脂肪酸，亚油酸（C18：2）和亚麻酸（C18：3）的组成增加，与葡萄糖相比。这表明甘露醇的氧化导致了R.河生植物Y2.这种脂肪酸组合物可有助于生物柴油燃料的冷流特性。我们的研究结果确定了一种具有独特代谢特征的耐盐产油酵母物种，证明了作为海洋生态系统全球碳循环中的分解者的关键作用。这是首次研究甘露醇诱导海洋酵母合成富含多不饱和脂肪酸的脂质。(C)2020爱思唯尔公司All rights reserved.
Brown macroalgae is a promising marine biomass for the production of bioethanol and biodiesel fuels. Here we investigate the biochemical processes used by marine oleaginous yeast for assimilating the major carbohydrate found in brown macroalgae. Briefly, yeast Rhodosporidiobolus fluvialis strain Y2 was isolated from seawater and grown in minimal medium containing reduced sugar alcohol mannitol as the sole carbon source with a salinity comparable to seawater. Conditions limiting nitrogen were used to facilitate lipid synthesis. R. fluvialis Y2 yielded 55.1% (w/w) and 39.1% (w/w) of lipids, per dry cell weight, from mannitol in the absence and presence of salinity, respectively. Furthermore, mannitol, as a sugar source, led to an increase in the composition of polyunsaturated fatty acids, linoleic acid (C18:2) and linolenic acid (C18:3), compared to glucose. This suggests that oxidation of mannitol leads to the activation of NADH-dependent fatty acid desaturases in R. fluvialis Y2. Such fatty acid composition may contribute to the cold-flow properties of biodiesel fuels. Our results identified a salt-tolerant oleaginous yeast species with unique metabolic traits, demonstrating a key role as a decomposer in the global carbon cycle through marine ecosystems. This is the first study on mannitol-induced synthesis of lipids enriched with polyunsaturated fatty acids by marine yeast. (C) 2020 Elsevier Inc. All rights reserved.