Fabrication and cellular compatibility of aligned chitosan-PCL fibers for nerve tissue regeneration
Fabrication and cellular compatibility of aligned chitosan-PCL fibers for nerve tissue regeneration
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DOI：
10.1016/j.carbpol.2011.02.008
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发表时间：
2011-04-22
影响因子：
11.2
通讯作者：
Zhang, Miqin
中科院分区：
文献类型：
--
作者：
Cooper, Ashleigh;Bhattarai, Narayan;Zhang, Miqin
生产排列的亚微米纤维的能力可能为开发用于组织工程和再生医学的支架开辟新的途径。一个特别感兴趣的领域是受损或患病神经的功能恢复，其中对齐的纤维用于支持细胞粘附和增殖，并引导神经突在纤维取向的方向上生长。在这项研究中，我们开发了一种对齐的壳聚糖-聚己内酯（壳聚糖-PCL）纤维支架，并研究了纤维对齐如何影响神经细胞的组织和功能，与随机取向的纤维支架和相同材料的流延膜相比。显示雪旺细胞（SC）在所有基底上附着和增殖，而不管其形貌如何，证明了壳聚糖-PCL材料的细胞相容性。在取向的壳聚糖-PCL纤维上生长的SC表现出沿纤维取向方向沿着取向的双极形态，而在膜和随机取向的纤维上生长的SC具有多极形态。类似地，壳聚糖-PCL材料支持神经元样PC-12细胞粘附，并且排列的纤维调节PC-12细胞沿着纤维取向的生长。此外，PC-12细胞培养在对齐的纤维上表现出增强的单向神经突延伸沿着纤维方向和显着更高的β-微管蛋白基因表达比那些生长在壳聚糖-PCL膜和随机取向的纤维。我们的研究表明，排列的壳聚糖-PCL纤维可以作为一个合适的支架，改善神经组织重建。(C)2011爱思唯尔有限公司保留所有权利。
The ability to produce aligned sub-micron fibers may open new avenues for the development of scaffolds for application in tissue engineering and regenerative medicine. An area of particular interest is functional restoration of damaged or diseased nerves where the aligned fibers serve to support cell adhesion and proliferation, and guide neurite outgrowth in the direction of fiber orientation. In this study, we developed an aligned chitosan-polycaprolactone (chitosan-PCL) fibrous scaffold and investigated how the fiber alignment influenced nerve cell organization and function in comparison with randomly oriented fibrous scaffolds and cast films of the same material. Schwann cells (SCs) were shown to attach and proliferate on all the substrates regardless of their topography, demonstrating the cellular compatibility of the chitosan-PCL material. SCs grown on the aligned chitosan-PCL fibers exhibited a bipolar morphology that oriented along the fiber alignment direction, while those on the films and randomly oriented fibers had a multipolar morphology. Similarly, the chitosan-PCL material supported neuron-like PC-12 cell adhesion, and the aligned fibers regulated the growth of PC-12 cells along the fiber orientation. Additionally, PC-12 cells cultured on the aligned fibers exhibited enhanced unidirectional neurite extension along fiber orientation and significantly higher beta-tubulin gene expression than those grown on chitosan-PCL films and randomly oriented fibers. Our investigation suggested that the aligned chitosan-PCL fibers can serve as a suitable scaffold for improved nerve tissue reconstruction. (C) 2011 Elsevier Ltd. All rights reserved.