Using the adult zebrafish visual system to study cadherin-2 expression during central nervous system regeneration.
Using the adult zebrafish visual system to study cadherin-2 expression during central nervous system regeneration.
复制标题
DOI：
10.1023/b:mics.0000006854.18378.fc
复制
发表时间：
2003-01-01
期刊：
影响因子：
--
通讯作者：
Londraville, R. L.
中科院分区：
文献类型：
--
作者：
Liu, Q.;Londraville, R. L.
斑马鱼是研究脊椎动物发育的良好模式生物，具有许多实验优势。在这里，我们表明，斑马鱼也适用于脊椎动物中枢神经系统组织再生的细胞和分子机制的研究。在这份报告中，我们描述了简单的协议，引入损伤的成年斑马鱼的视觉结构和分析钙粘蛋白-2表达的再生视觉系统，使用免疫细胞化学和免疫印迹。
Zebrafish is a good model organism for the study of vertebrate development due to numerous experimental advantages. Here we show that zebrafish are also suitable for the study of cellular and molecular mechanisms underlying tissue regeneration in the vertebrate central nervous system. In this report we describe simple protocols to introduce injuries to the adult zebrafish visual structures and analyze cadherin-2 expression in the regenerating visual system using immunocytochemistry and immunoblotting.