Capillary electrophoretic separation of uncharged polymers using polyelectrolyte engines - Theoretical model
Capillary electrophoretic separation of uncharged polymers using polyelectrolyte engines - Theoretical model
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DOI：
10.1016/s0021-9673(01)00990-6
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发表时间：
2001-07-27
影响因子：
4.1
通讯作者：
Drouin, G
中科院分区：
文献类型：
--
作者：
McCormick, LC;Slater, GW;Drouin, G
我们最近证明，可以使用 DNA-聚合物缀合物的自由溶液毛细管电泳来分析不带电聚合物样品的分子量分布。在这些缀合物中，DNA 提供电动势，而样品的不带电多分散聚合物链则以不同量的流体动力阻力阻碍 DNA 引擎。在这里，我们提出了这种新分析方法的理论模型。我们表明，对于最有利的扩散限制电泳条件，实际上存在实现给定聚合物样品分离的最佳 DNA 大小。此外，我们证明聚合物链的有效摩擦系数与缀合物的两种聚合物的刚度有关，从而提供了一种通过迁移率测量来估计不带电聚合物的持久长度的方法。最后，我们将一些预测与可用的实验结果进行比较。 (C) 2001 Elsevier Science B.V. 保留所有权利。
We recently demonstrated that the molecular mass distribution of an uncharged polymer sample can be analyzed using free-solution capillary electrophoresis of DNA-polymer conjugates. In these conjugates, the DNA is providing the electromotive force while the uncharged polydisperse polymer chains of the sample retard the DNA engine with different amounts of hydrodynamic drag. Here we present a theoretical model of this new analytical method. We show that for the most favourable, diffusion-limited electrophoresis conditions, there is actually an optimal DNA size to achieve the separation of a given polymer sample. Moreover, we demonstrate that the effective friction coefficient of the polymer chains is related to the stiffness of the two polymers of the conjugate, thus offering a method to estimate the persistence length of the uncharged polymer through mobility measurements. Finally, we compare some of our predictions with available experimental results. (C) 2001 Elsevier Science B.V. All rights reserved.