OY-TES-1 may regulate the malignant behavior of liver cancer via NANOG, CD9, CCND2 and CDCA3: A bioinformatic analysis combine with RNAi and oligonucleotide microarray
OY-TES-1 may regulate the malignant behavior of liver cancer via NANOG, CD9, CCND2 and CDCA3: A bioinformatic analysis combine with RNAi and oligonucleotide microarray
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OY-TES-1可能通过NANOG、CD9、CCND2和CDCA3调节肝癌的恶性行为：结合RNAi和寡核苷酸微阵列的生物信息学分析
DOI：
10.3892/or.2015.3792
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发表时间：
2015-04-01
期刊：
影响因子：
4.2
通讯作者：
Xiao, Shaowen
中科院分区：
文献类型：
--
作者：
Hu, Qiping;Fu, Jun;Xiao, Shaowen
鉴于其肿瘤特异性表达，包括在肝癌中的表达，OY - TES - 1是肝癌诊断和免疫治疗的一个潜在分子标志物。然而，关于OY - TES - 1在肝癌中的作用机制的研究却很少。在本研究中，基于综合生物信息学分析，并结合RNA干扰（RNAi）和寡核苷酸微阵列技术，我们首次报道在肝癌细胞系BEL - 7404中，OY - TES - 1的下调导致NANOG、CD9、CCND2和CDCA3的表达发生显著变化。NANOG、CD9、CCND2和CDCA3可能参与细胞增殖、迁移、侵袭和凋亡，并且它们之间以及与OY - TES - 1可能存在功能上的关联。在这些分子中，我们发现含有Kazal - 2结合基序和同源框的NANOG可能是在肝癌中与OY - TES - 1相互作用的最有可能的候选蛋白。因此，本研究可能为进一步研究OY - TES - 1在肝癌中的作用提供重要信息。
Given its tumor-specific expression, including liver cancer, OY-TES-1 is a potential molecular marker for the diagnosis and immunotherapy of liver cancers. However, investigations of the mechanisms and the role of OY-TES-1 in liver cancer are rare. In the present study, based on a comprehensive bioinformatic analysis combined with RNA interference (RNAi) and oligonucleotide microarray, we report for the first time that downregulation of OY-TES-1 resulted in significant changes in expression of NANOG, CD9, CCND2 and CDCA3 in the liver cancer cell line BEL-7404. NANOG, CD9, CCND2 and CDCA3 may be involved in cell proliferation, migration, invasion and apoptosis, yet also may be functionally related to each other and OY-TES-1. Among these molecules, we identified that NANOG, containing a Kazal-2 binding motif and homeobox, may be the most likely candidate protein interacting with OY-TES-1 in liver cancer. Thus, the present study may provide important information for further investigation of the roles of OY-TES-1 in liver cancer.