Effects of Taxol on the developmental potency of human and mouse GV oocytes
Effects of Taxol on the developmental potency of human and mouse GV oocytes
复制标题
紫杉醇对人和小鼠 GV 卵母细胞发育效能的影响
DOI：
10.1093/humrep/deac106.082
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发表时间：
2022
影响因子：
6.1
通讯作者：
Atsushi T
中科院分区：
文献类型：
--
作者：
Watanabe S;Atsushi T
研究问题：是否有可能提高延迟成熟或老化卵母细胞的质量？摘要答案是的，在生殖泡（GV）阶段修饰卵母细胞微管可以抑制体外成熟中染色体的异常分离，促进早期卵裂。已知卵母细胞老化的特征是随着母体年龄的增长，非整倍体增加，发育潜能下降，这将导致低频率的胚细胞形成和植入。除了第一次减数分裂之外，已经清楚的是，第二次减数分裂也显著有助于非整倍体产生，随后是植入前胚胎丢失。研究设计、大小、持续时间使用从同意的女性患者中获得的具有延迟成熟的人GV卵母细胞。还使用从9-15月龄ICR小鼠收集的小鼠GV卵母细胞。用Taxol处理1hr后，体外成熟的卵母细胞，分别在GV期、MII期和原核期进行细胞遗传学和细胞学检查，采用基因芯片技术比较Taxol处理前后两个物种GV期卵母细胞的RNA转录水平。在人类IVM卵母细胞中检测MII期染色体畸变和囊胚形成率。小鼠IVM卵母细胞在第二极体（PB）挤出和O2消耗（CRAS 3.0，Crino Co Inc.）主要结果和机会的作用经泰素处理后，人的染色体分离率从96%降低到7%，囊胚形成率从3%提高到16%。在小鼠中，正常PB排出率从29%增加到92%，并且在某些原核卵母细胞中耗氧量更高。在人GV期卵母细胞中，紫杉醇处理仅改变了与染色体附着和分离相关的少数因素的基因表达。另一方面，在小鼠GV卵母细胞，基因表达显着改变卵母细胞老化和紫杉醇treatment.Limitations，causes for cautionThe目前的结果是在人GV卵母细胞与成熟延迟后收集PMS和HCG管理。目前尚不清楚Taxol对注射HCG前收集的GV期卵母细胞是否有效。本研究结果的更广泛意义我们的研究结果表明，在GV期结合生化修饰和IVM可能能够恢复人类卵母细胞的老化。试验注册号不适用
Study questionIs it possible to improve the quality of oocytes with delayed maturation or aging?Summary answerYes, modification of oocyte microtubules at the germinal vesicle (GV) stage inhibits abnormal chromosome separation in in-vitro maturation and enhances early cleavage.What is known alreadyOocyte aging is characterized by an increase of aneuploidy and a decrease of the developmental potency with maternal ages, which will results in low frequencies of the blastocyte formation and implantation. In addition to the 1st meiotic division, it has become clear that the 2nd meiotic division also significantly contributes to aneuploidy production which is followed by pre-implantation embryo loss. However, no method for overcoming the oocyte aging has been established.Study design, size, durationHuman GV oocytes with delayed maturation obtained from consented female patients were used. Mouse GV oocytes collected from 9-15 month old ICR mice were also used. After exposure to Taxol for 1hr, the oocytes were maturedin vitroand examined cytogenetically and cytologically at the GV stage, the MII stage and pronuclear stage.Participants/materials, setting, methodsRNA transcripts of the GV stage oocytes were compared before and after Taxol exposure with microarray in both species. Chromosome aberrations at the MII stage and blastocyst formation rate were examined in human IVM oocytes. Mouse IVM oocytes were evaluated on 2nd polar body (PB) extrusion and O2consumption (CRAS3.0, Crino Co Inc.) at the pronuclear stage after parthenogenetic activation with electro-stimulation.Main results and the role of chanceAfter Taxol treatment, premature chromosome separation was significantly reduced from 96% to 7% and the blastocyst formation rate increased from 3% to 16% in human. In mice, normal PB extrusion rate increased from 29% to 92%, and oxygen consumption was higher in some pronuclear oocytes. In human GV oocytes, taxol treatment altered gene expression only in a few factors related to chromosome attachment and segregation. On the other hand, in mouse GV oocytes, gene expression was significantly altered by oocyte aging and Taxol treatment.Limitations, reasons for cautionThe present results were obtained in human GV oocytes with delayed maturation which were collected after PMS and HCG administrations. It is not clear whether Taxol is effective on the GV oocytes collected before HCG administration.Wider implications of the findingsOur results indicate that the combination of biochemical modification and IVM in the GV stage may be able to restore the aging of human oocytes.Trial registration numbernot applicable