Migration of Phospholipid Vesicles Can Be Selectively Driven by Concentration Gradients of Metal Chloride Solutions
Migration of Phospholipid Vesicles Can Be Selectively Driven by Concentration Gradients of Metal Chloride Solutions
复制标题
金属氯化物溶液的浓度梯度可以选择性地驱动磷脂囊泡的迁移
DOI：
10.1021/acs.langmuir.7b02617
复制
发表时间：
2017
期刊：
影响因子：
3.9
通讯作者：
and Miglena I. Angelova
中科院分区：
文献类型：
--
作者：
Atsuji Kodama;Yuka Sakuma;Masayuki Imai;Toshihiro Kawakatsu;Nicolas Puff;and Miglena I. Angelova
我们研究了金属离子浓度梯度下磷脂囊泡的迁移。我们将一价（NaCl和KCl）、二价（CaCl 2和MgCl 2）和三价（LaCl 3）金属氯化物溶液显微注射到由1，2-二油酰-sn-甘油-3-磷酸胆碱（DOPC）组成的磷脂巨囊泡（GV）中。对于NaCl、CaCl 2和MgCl 2溶液，GV响应于浓度梯度直接向微量移液器的尖端迁移，而对于KCl和LaCl 3，GV向相反的方向移动。我们的运动跟踪的脂质结构域在囊泡膜显示没有单向流动的膜在囊泡迁移过程中，表明马兰戈尼机制是不负责所观察到的囊泡迁移。通过数值求解斯托克斯方程，计算了对称和非对称电解质的扩散电泳速度。理论扩散电泳速度很好地描述了观察到的迁移速度。因此，我们可以通过调节电解质和脂质来控制囊泡响应浓度梯度的迁移。
We have investigated the migrations of phospholipid vesicles under the concentration gradients of metal ions. We microinjected metal chloride solutions, monovalent (NaCl and KCl), divalent (CaCl2and MgCl2), and trivalent (LaCl3) salts, toward phospholipid giant vesicles (GVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC). For NaCl, CaCl2, and MgCl2solutions, the GVs migrated straight toward the tip of the micropipette in response to the concentration gradients, whereas for KCl and LaCl3, GVs moved to the opposite direction. Our motion tracking of lipid domains in a vesicle membrane showed no unidirectional flow in the membrane during the vesicle migration, indicating that the Marangoni mechanism is not responsible for the observed vesicle migration. We calculated the diffusiophoretic velocities for symmetric and asymmetrical electrolytes by solving the Stokes’ equation numerically. The theoretical diffusiophoretic velocities described the observed migration velocities well. Thus, we can control the migration of vesicle in response to the concentration gradient by adapting the electrolytes and the lipids.