Plasmid segregation into minicells is associated with membrane attachment and independent of plasmid replication.
Plasmid segregation into minicells is associated with membrane attachment and independent of plasmid replication.
复制标题
质粒分离到小细胞中与膜附着相关并且与质粒复制无关。
DOI：
10.1128/jb.165.1.82-87.1986
复制
发表时间：
1986
影响因子：
3.2
通讯作者：
Levy,SB
中科院分区：
文献类型：
--
作者：
Rashtchian,A;Brown,SW;Reichler,J;Levy,SB
The role of plasmid replication in the segregation of plasmids into Escherichia coli minicells was investigated with temperature-sensitive replication mutants derived from E. coli plasmids ColE1 and pSC101. For as long as six generations of growth, at permissive or nonpermissive temperatures (when greater than 80% of plasmid replication was inhibited), the same amount of previously 3H-labeled plasmid DNA segregated into minicells. Density gradient separations of wild-type and temperature-sensitive plasmid DNA from both replicons segregated into the minicells showed that about 20 to 25% was stably associated with the minicell membrane at both temperatures. Electron microscopy showed this DNA to consist of circular plasmid molecules attached to the minicell membrane. These combined findings suggest that segregation of plasmids into minicells and their association with the minicell membrane are interrelated and independent of plasmid replication.
