Death of female flower microsporocytes progresses independently of meiosis-like process and can be accelerated by specific transcripts in Asparagus officinalis
Death of female flower microsporocytes progresses independently of meiosis-like process and can be accelerated by specific transcripts in Asparagus officinalis
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DOI：
10.1038/s41598-019-39125-1
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发表时间：
2019-02
影响因子：
4.6
通讯作者：
M. Ide;K. Masuda;D. Tsugama;K. Fujino
中科院分区：
文献类型：
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作者：
M. Ide;K. Masuda;D. Tsugama;K. Fujino
芦笋（花园芦笋）是一种雌雄异株的多年生作物，A.药用成分会影响其生产力。因A。在药用植物中，雌性植物的花药无法在小孢子母细胞周围积累胼胝质，无法完成减数分裂，并因细胞死亡而退化。尽管有 13 个基因与雄花和雌花的花药发育有关，但尚不清楚这些基因如何调节雌花花药的细胞死亡。本研究的目的是缩小这一过程中涉及的因素范围。雌花小孢子母细胞的TUNEL染色和Feulgen染色表明雌性小孢子母细胞进入了先前未检测到的减数分裂样过程，并且细胞死亡独立于这种减数分裂样过程而发生，排除了细胞死亡是由减数分裂停止引起的可能性。对单个花器官（花被片、雌蕊和雄蕊）的 RNA 测序表明，一些可能调节细胞死亡的基因，如元半胱天冬酶基因和 Bax 抑制剂-1 基因，在雌花和雄花花药之间受到差异性调节，并且参与胼胝质积累的基因仅在雄花花药中上调。这些基因可能参与调节 A 中雌花花药的细胞死亡。药用植物。
Asparagus officinalis(garden asparagus) is a dioecious perennial crop, and the dioecy (i.e., sex) ofA. officinaliscan affect its productivity. InA. officinalis, flower anthers in female plants fail to accumulate callose around microsporocytes, fail to complete meiosis, and degenerate due to cell death. Although 13 genes have been implicated in the anther development of male and female flowers, it is unclear how these genes regulate the cell death in female flower anthers. The aim of this study was to narrow down factors involved in this process. TUNEL staining and Feulgen staining of female flower microsporocytes suggest that female microsporocytes enter a previously undetected meiosis-like process, and that the cell death occurs independently of this meiosis-like process, excluding the possibility that the cell death is caused by the cessation of meiosis. RNA sequencing with individual floral organs (tepals, pistils and stamens) revealed that several genes possibly regulating the cell death, such as metacaspase genes and a Bax inhibitor-1 gene, are differentially regulated between female and male flower anthers, and that genes involved in callose accumulation are up-regulated only in male flower anthers. These genes are likely involved in regulating the cell death in female flower anthers inA. officinalis.