Interaction of von Willebrand factor with human platelets in the plasma milieu.
Interaction of von Willebrand factor with human platelets in the plasma milieu.
复制标题
血管性血友病因子与血浆环境中人血小板的相互作用。
DOI：
10.1172/jci111228
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Montgomery,RR
中科院分区：
文献类型：
--
作者：
Schullek,J;Jordan,J;Montgomery,RR
使用放射性标记的vWf单克隆抗体进行血浆环境中血管性血友病因子（vWf）与刺激血小板的结合。血浆蛋白特异性抑制凝血酶和ADP/肾上腺素诱导的vWf与活化血小板的结合，但不抑制瑞斯托菌素诱导的vWf结合。当正常血浆被热脱乙酰基时，单克隆标记的vWf在凝血酶或ADP/肾上腺素刺激后与血小板结合。此外，来自无纤维蛋白原血症血浆的单克隆标记的vWf与血小板正常结合。vWf与热脱乙酰正常血浆或无纤维蛋白原血症血浆中的刺激血小板的结合被以浓度依赖性方式加入正常血浆纤维蛋白原特异性抑制。然而，在纤维蛋白原低于1 mg/ml的水平下，可以证明vWf结合。vWf与血小板结合的纤维蛋白原的抑制是竞争性的，并通过增加vWf的浓度来克服。这些研究表明，凝血酶诱导的和ADP/肾上腺素诱导的vWf与血小板的结合在血浆环境中不会发生，尽管在纤维蛋白原水平降低时，vWf与刺激的血小板的结合可以被证明。图片
The binding of von Willebrand factor (vWf) to stimulated platelets in the plasma milieu was performed using a radiolabeled monoclonal antibody to vWf. Plasma proteins specifically inhibited the thrombin- and ADP/epinephrine-induced vWf binding to activated platelets but did not inhibit the ristocetin-induced vWf binding. When normal plasma was heat defibrinated, monoclonal-labeled vWf was bound to platelets following thrombin or ADP/epinephrine stimulation. Furthermore, monoclonal-labeled vWf from afibrinogenemic plasma bound normally to platelets. The binding of vWf to stimulated platelets in either heat-defibrinated normal plasma or afibrinogenemic plasma was specifically inhibited by the addition of normal plasma fibrinogen in a concentration-dependent manner. At levels of fibrinogen less than 1 mg/ml, however, vWf binding could be demonstrated. The inhibition by fibrinogen of vWf binding to platelets was competitive and overcome by increased concentrations of vWf. These studies show that thrombin-induced and ADP/epinephrine-induced vWf binding to platelets does not occur in the plasma milieu, although at reduced levels of fibrinogen, vWf binding to stimulated platelets can be demonstrated.Images