Quantitative analysis of amylopectin unit chains by means of high-performance anion-exchange chromatography with pulsed amperometric detection
Quantitative analysis of amylopectin unit chains by means of high-performance anion-exchange chromatography with pulsed amperometric detection
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DOI：
10.1016/s0021-9673(97)01151-5
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发表时间：
1998-03-27
影响因子：
4.1
通讯作者：
Åman, P
中科院分区：
文献类型：
--
作者：
Koch, K;Andersson, R;Åman, P
高效阴离子交换色谱-脉冲安培检测（HPAEC-PAD）是一种广泛应用于支链淀粉链长分布研究的技术。然而，色谱图不能直接反映这种分布，因为PAD响应随聚合度而变化。在这项研究中，糯玉米淀粉脱支和分级的生物凝胶P-6柱和响应因子的麦芽糖与DP 3-65测定。对于DP 3-7，每微克葡聚糖链的检测器响应显示出显著降低，而对于DP大于15，曲线变平。(C)1998年Elsevier Science B.V.
High-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) is a widely used technique to study the chain length distribution of amylopectin. The chromatograms, however, do not directly reflect this distribution, since the PAD response changes with the degree of polymerization. In this study, waxy maize starch was debranched and fractionated on a Bio-Gel P-6 column and the response factors for maltosaccharides with DP 3-65 were determined. The detector response per mu g glucan chains was shown to decrease considerably for DP 3-7 while the curve leveled out for DP larger than 15. (C) 1998 Elsevier Science B.V.