Adenosine phosphorylase-mediated nucleoside toxicity. Application towards the detection of mycoplasmal infection in mammalian cell cultures.
Adenosine phosphorylase-mediated nucleoside toxicity. Application towards the detection of mycoplasmal infection in mammalian cell cultures.
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腺苷磷酸化酶介导的核苷毒性。
DOI：
10.1016/0014-4827(82)90333-0
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发表时间：
1982
影响因子：
3.7
通讯作者：
Carson,DA
中科院分区：
文献类型：
--
作者：
McGarrity,GJ;Carson,DA
腺苷磷酸化酶（腺苷：正磷酸核糖基转移酶）活性在哺乳动物细胞中很低，但在某些原核生物中很丰富。它是开发诊断和化疗药物的合适靶酶。通常感染细胞培养物的枯草芽孢杆菌和支原体的腺苷磷酸化酶将无毒的脱氧腺苷类似物6-甲基嘌呤脱氧核苷（6-methylpurine deoxyriboside，6 MPDR）转化为有效的抗代谢物6-甲基嘌呤和6-甲基嘌呤核苷。因此，6 MPDR选择性地杀死感染支原体的哺乳动物细胞培养物。6 MPDR对90株支原体感染培养物中87株（96.6%）有细胞毒性。在9种不同类型的无支原体细胞培养物中未观察到毒性。使用3 T6小鼠胚胎成纤维细胞指示细胞培养提高了标准化。将10 μM 6 MPDR接种至感染以下支原体的3 T6培养物后3-4天，细胞毒性明显：M. hyorhinis、猪鼻霉M. orale，M. M.C. salivarium，莱氏细柄菊A，M. hominis，M. fermentons，M. sp.70 -159和M.颊部在存在10 μM 6 MPDR的情况下，在接种支原体感染细胞培养物的28/28 3 T6指示培养物中产生细胞毒性。该类似物对支原体明显无毒。
Adenosine phosphorylase (adenosine: orthophosphate ribosyltransferase) activity is low in mammalian cells, but is abundant in certain prokaryotes. It is an appropriate target enzyme for the development of diagnostic and chemotherapeutic agents. Adenosine phosphorylase fromBacillus subtilisand mycoplasmas that commonly infect cell cultures converted the non-toxic deoxyadenosine analog, 6-methylpurine deoxyriboside (6MPDR) into the potent anti-metabolites 6-methylpurine and 6-methylpurine riboside. Consequently 6MPDR selectively killed mammalian cell cultures infected with mycoplasmas. 6MPDR was cytotoxic to 87 of 90 mycoplasma-infected cultures (96.6%). No toxicity was observed in nine different types of mycoplasma-free cell cultures. Use of a 3T6 mouse embryo fibroblast indicator cell culture improved standardization. Cytotoxicity was apparent 3–4 days after inoculation of 10 μM of 6MPDR to 3T6 cultures infected with the following mycoplasmas:M. hyorhinis, M. orale, M. arginini, M. salivarium, A, laidlawii, M. hominis, M. fermentons, M. sp. 70–159 andM. buccale. Cytotoxicity was produced in 28/28 3T6 indicator cultures inoculated with mycoplasma-infected cell cultures in the presence of 10 μM 6MPDR. The analog is apparently non-toxic to the mycoplasmas.