Fibroblast Growth Factor-2 Enhances Expansion of Human Bone Marrow-Derived Mesenchymal Stromal Cells without Diminishing Their Immunosuppressive Potential.
Fibroblast Growth Factor-2 Enhances Expansion of Human Bone Marrow-Derived Mesenchymal Stromal Cells without Diminishing Their Immunosuppressive Potential.
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DOI：
10.4061/2011/235176
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发表时间：
2011-03-03
影响因子：
4.3
通讯作者：
Solchaga LA
中科院分区：
文献类型：
--
作者：
Auletta JJ;Zale EA;Welter JF;Solchaga LA
同种异体造血干细胞移植是许多血液系统恶性肿瘤的主要治疗方法。其潜力依赖于与移植物抗宿主病相关的移植物抗肿瘤效应。间充质间质细胞（MSCs）具有免疫调节特性，使其成为有吸引力的治疗选择。我们评估了由来自5个供体的人间充质干细胞调节的培养基的体外免疫抑制活性，该培养基扩增了13代，含或不含FGF-2。在第7代和第13代中，FGF-2的补充分别使扩增量增加了3500倍和24万倍。在不同条件下扩增的传代匹配细胞培养基之间的免疫抑制活性没有差异，但fgf处理的MSCs培养基优于群体双匹配对照。三种制剂的免疫抑制活性保持不变，但两种制剂的免疫抑制活性随扩增而降低。FGF-2诱导的增殖不导致免疫抑制活性的丧失。然而，由于免疫抑制活性不能始终保持，因此必须谨慎行事，以确保细胞在广泛扩增后具有足够的活性。
Allogeneic hematopoietic stem cell transplantation is the main curative therapy for many hematologic malignancies. Its potential relies on graft-versus-tumor effects which associate with graft-versus-host disease. Mesenchymal stromal cells (MSCs) possess immunomodulatory properties that make them attractive therapeutic alternatives. We evaluated the in vitro immunosuppressive activity of medium conditioned by human MSCs from 5 donors expanded 13 passages with or without FGF-2. FGF-2 supplementation increased expansion 3,500- and 240,000-fold by passages 7 and 13, respectively. There were no differences in immunosuppressive activity between media conditioned by passage-matched cells expanded under different conditions, but media conditioned by FGF-treated MSCs were superior to population doubling-matched controls. The immunosuppressive activity was maintained in three of the preparations but decreased with expansion in two. The proliferation induced by FGF-2 did not result in loss of immunosuppressive activity. However, because the immunosuppressive activity was not consistently preserved, caution must be exercised to ensure that the activity of the cells is sufficient after extensive expansion.
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影响因子：
2.2
作者：
CZERKINSKY, CC;NILSSON, LA;TARKOWSKI, A
通讯作者：
TARKOWSKI, A
影响因子：
20.3
作者：
Aggarwal, S;Pittenger, MF
通讯作者：
Pittenger, MF
影响因子：
4.8
作者：
Jaiswal, RK;Jaiswal, N;Pittenger, MF
通讯作者：
Pittenger, MF
影响因子：
168.9
作者：
LeBlanc, Katarina;Frassoni, Francesco;Ringden, Olle
通讯作者：
Ringden, Olle
影响因子：
3.7
作者：
Bianchi, G;Banfi, A;Quarto, R
通讯作者：
Quarto, R