Microbial communities in freshwater used for hydraulic fracturing are unable to withstand the high temperatures and pressures characteristic of fractured shales
Microbial communities in freshwater used for hydraulic fracturing are unable to withstand the high temperatures and pressures characteristic of fractured shales
复制标题
用于水力压裂的淡水中的微生物群落无法承受裂隙页岩的高温和高压特征
DOI:
10.1099/acmi.0.000515.v1
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发表时间:
2022
期刊:
影响因子:
--
通讯作者:
Nixon S
中科院分区:
文献类型:
--
作者:
Nixon S
Natural gas is recovered from shale formations by hydraulic fracturing, a process known to create microbial ecosystems in the deep subsurface. Microbial communities that emerge in fractured shales include organisms known to degrade fracturing fluid additives and contribute to corrosion of well infrastructure. In order to limit these negative microbial processes, it is essential to constrain the source of the responsible micro-organisms. Previous studies have identified a number of potential sources, including fracturing fluids and drilling muds, yet these sources remain largely untested. Here, we apply high-pressure experimental approaches to assess whether the microbial community in synthetic fracturing fluid made from freshwater reservoir water can withstand the temperature and pressure conditions of hydraulic fracturing and the fractured shale environment. Using cell enumerations, DNA extraction and culturing, we show that the community can withstand high pressure or high temperature alone, but the combination of both is fatal. These results suggest that initial freshwater-based fracturing fluids are an unlikely source of micro-organisms in fractured shales. These findings indicate that potentially problematic lineages, such as sulfidogenic strains ofHalanaerobiumthat have been found to dominate fractured shale microbial communities, likely derive from other input sources into the downwell environment, such as drilling muds.
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DOI:
10.1038/ismej.2012.8
发表时间:
2012-08
期刊:
The ISME journal
影响因子:
--
作者:
通讯作者:
--
影响因子:
4.6
作者:
S. Nixon;B. V. van Dongen;C. Boothman;J. Small;J. Lloyd
通讯作者:
J. Lloyd
影响因子:
3.9
作者:
通讯作者:
--
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
K. S. Johnson;K. French;J. Fichter;R. Oden
通讯作者:
R. Oden
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
S. L. Moore;C. Cripps
通讯作者:
C. Cripps