Sinorhodobacter hungdaonensis sp nov isolated from activated sludge collected from a municipal wastewater treatment plant
Sinorhodobacter hungdaonensis sp nov isolated from activated sludge collected from a municipal wastewater treatment plant
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从城市污水处理厂收集的活性污泥中分离出洪道中华红细菌 nov
DOI：
10.1007/s10482-016-0770-x
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发表时间：
2017
影响因子：
2.6
通讯作者：
Li Jing
中科院分区：
文献类型：
--
作者：
Xi Lijun;Qiao Nenghu;Zhang Zhenchong;Yan Laihong;Li Fengbo;Hu Jinpeng;Li Jing
从中国黄岛市污水处理厂的活性污泥样品中分离出一种新细菌，菌株 L3T。根据16S rRNA基因序列相似性研究，菌株L3T隶属于中华红细菌属，与铁还原中华红细菌属关系最密切(98.0%)。菌株L3T与其他相关物种、Thioclava atlanticaDLFJ1-1T(96.5 %)、Rhodobacter capsulatus ATCC 11166T(96.3 %)、Paenirhodobacter enshiensisDW2-9T(96.3 %)和Rhodobacter的16S rRNA基因序列相似性 viridisJA737T(96.0%) 小于 96.5%。化学分类学特征进一步支持该菌株属于中华红细菌属。主要的极性脂质由二磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺组成。主要脂肪酸是 C18:1ω7c (66.3%)、C16:0(12.9%) 和 C18:0(8.0%)。主要醌是Q-10。菌株L3Ti基因组DNA的G+C含量为68.0mol%。 L3T与密切相关型菌株S之间的DNA-DNA相关性值。 ferrireducensSgZ-3T 为 35.2%。基于这些结果，提出了一个新种黄岛中华红细菌。模式菌株为 L3T（= CGMCC 1.12963T= KCTC 42823T）。
A novel bacterium, strain L3T, was isolated from an activated sludge sample retrieved from a municipal wastewater treatment plant in Huangdao, China. On the basis of 16S rRNA gene sequence similarity studies, strain L3Twas affiliated to the genusSinorhodobacter, being most closely related toSinorhodobacter ferrireducens(98.0 %). The 16S rRNA gene sequence similarity of strain L3Tto other related species,Thioclava atlanticaDLFJ1-1T(96.5 %),Rhodobacter capsulatusATCC 11166T(96.3 %),Paenirhodobacter enshiensisDW2-9T(96.3 %) andRhodobacter viridisJA737T(96.0 %) is less than 96.5 %. Chemotaxonomic characterization further supported classification of the strain to the genusSinorhodobacter. The major polar lipid profile consists of diphosphatidyglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major fatty acids are C18:1ω7c (66.3 %), C16:0(12.9 %) and C18:0(8.0 %). The major quinone is Q-10. The G+C content of the genomic DNA of strain L3Tis 68.0 mol %. DNA–DNA relatedness value between L3Tand the closely related type strainS. ferrireducensSgZ-3Twas 35.2 %. Based on these results, a new speciesSinorhodobacter huangdaonensisis proposed. The type strain is L3T(= CGMCC 1.12963T= KCTC 42823T).