Transfer of macrophage-derived mycobacterial antigens to dendritic cells can induce naive T-cell activation
Transfer of macrophage-derived mycobacterial antigens to dendritic cells can induce naive T-cell activation
复制标题
DOI：
10.1046/j.1365-3083.2003.01191.x
复制
发表时间：
2003-02-01
影响因子：
3.7
通讯作者：
Baird, MA
中科院分区：
文献类型：
--
作者：
Girvan, A;Aldwell, FE;Baird, MA
分枝杆菌能够在宿主巨噬细胞中存活和复制，它们可以将抗原物质释放到环境中。然而，与树突状细胞（dc）不同，巨噬细胞似乎不能激活幼稚T细胞。因此，本研究研究了巨噬细胞与DCs之间的抗原转移。我们从卡介苗感染和未感染的巨噬细胞中提取培养上清液，然后测定DCs是否能将这些细胞外分枝杆菌抗原呈递到T细胞。在这里，我们证明了用bcg感染巨噬细胞释放的抗原脉冲的dc可以在体外刺激启动T细胞，并在体内启动初始T细胞反应。这些结果表明抗原可以在巨噬细胞和dc之间发生转移。
Mycobacteria are capable of surviving and replicating in host macrophages, where they can release antigenic material into the environment. However, unlike dendritic cells (DCs), macrophages do not appear to be capable of activating naive T cells. Therefore, this work investigated antigen transfer between macrophages and DCs. We generated culture supernatants from bacille Calmette-Guerin (BCG)-infected and uninfected macrophages and then determined whether DCs could present these extracellular mycobacterial antigens to T cells. Here, we show that DCs pulsed with antigens released from BCG-infected macrophages can stimulate primed T cells in vitro and initiate naive T-cell responses in vivo . These results suggest that antigen transfer can occur between macrophages and DCs.