A rice FRD3-like (OsFRDL1) gene is expressed in the cells involved in long-distance transport
A rice FRD3-like (OsFRDL1) gene is expressed in the cells involved in long-distance transport
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DOI：
10.1080/00380768.2004.10408586
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发表时间：
2004-12-01
影响因子：
2
通讯作者：
Nishizawa, NK
中科院分区：
文献类型：
--
作者：
Inoue, H;Mizuno, D;Nishizawa, NK
我们在水稻基因组中鉴定了4个与拟南芥FRD 3具有39.1 ~ 56.7%氨基酸序列相似性的AtFRD 3样基因（OsFRDL）。其中，我们从缺铁水稻根制备的cDNA文库中克隆了三个OsFRDL基因：OsFRDL 1、OsFRDL 2和OsFRDL 3。OsFRDL 1在铁营养充足的根中表达较弱，在缺铁的根中表达较弱。OsFRDL 2在根和叶中组成型表达，缺铁降低了其在叶中的表达。OsFRDL 3在叶片中表达，在根中不表达;缺铁诱导叶片中表达量较低。OsFRDL 1-sGFP融合蛋白定位于洋葱表皮细胞的质膜。启动子GUS分析表明，OsFRDL 1定位于参与远距离运输的细胞，在铁充足和铁缺乏的植物。此外，OsFRDL 1在生殖阶段也有表达。这些结果表明，OsFRDL 1是一种位于细胞质膜上的转运蛋白，参与远距离转运。
We identified four putative AtFRD3-like genes (OsFRDL) in the rice genome that exhibited 39.1 to 56.7% amino acid sequence similarities to Arabidopsis FRD3. Of these, we cloned three OsFRDL genes from a cDNA library prepared from iron-deficient rice roots: OsFRDL1, OsFRDL2, and OsFRDL3. OsFRDL1 was expressed weakly in Fe-sufficient roots, and slight expression was induced in the roots of Fe-deficient plants. OsFRDL2 was expressed constitutively in both roots and leaves, and Fe deficiency reduced its expression in leaves. OsFRDL3 was expressed in leaves, but not in roots; Fe deficiency induced slight expression in leaves. An OsFRDL1-sGFP fusion protein was localized in the plasma membrane in onion epidermal cells. The promoter GUS analysis showed that OsFRDL1 was localized in the cells involved in long-distance transport, in both Fe-sufficient and Fe-deficient plants. Furthermore, OsFRDL1 expression was observed during the reproductive stage. These results suggest that OsFRDL1 is a transporter that resides in the plasma membrane of cells involved in long-distant transport.