Comparison of anode bacterial communities and performance in microbial fuel cells with different electron donors
Comparison of anode bacterial communities and performance in microbial fuel cells with different electron donors
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DOI：
10.1007/s00253-007-1162-y
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发表时间：
2007-11-01
影响因子：
5
通讯作者：
Regan, John M.
中科院分区：
文献类型：
--
作者：
Jung, Sokhee;Regan, John M.
微生物燃料电池（MFC）利用某些微生物的电化学活性，从还原的化合物中产生电力。MFC阳极生物膜的表征共同表现出非常多样化的微生物群落，提出了这些阳极还原群落内的竞争和群落演替的生态问题。三组三重，两室的MFC接种厌氧污泥和不同的能源（乙酸，乳酸，和葡萄糖）进行了操作，以探讨这些问题。基于16 S rDNA-靶向变性梯度凝胶电泳（DGGE），所有阳极群落包含与还原硫地孕酮密切相关的序列（> 99%相似性）和拟杆菌纲中的未培养细菌克隆（99%相似性）。在大多数阳极生物膜中也富集了各种其他类地细菌序列。虽然每个基板的复制反应器中的阳极社区一般收敛到一个可再生的社区，有一些变化，这些假定的阳极还原类地细菌菌株的相对分布。厚壁菌只在葡萄糖喂养的MFC中发现，推测其作用是将复杂的碳转化为简单的分子并清除氧气。这些系统中的最大电流密度与重复反应器之间的内阻变化呈负相关，并且可能仅受这些具有高内阻的两室MFC中的阳极群落差异的最小影响。
Microbial fuel cells (MFCs) harness the electrochemical activity of certain microbes for the production of electricity from reduced compounds. Characterizations of MFC anode biofilms have collectively shown very diverse microbial communities, raising ecological questions about competition and community succession within these anode-reducing communities. Three sets of triplicate, two-chamber MFCs inoculated with anaerobic sludge and differing in energy sources (acetate, lactate, and glucose) were operated to explore these questions. Based on 16S rDNA-targeted denaturing gradient gel electrophoresis (DGGE), all anode communities contained sequences closely affiliated with Geobacter sulfurreducens (> 99% similarity) and an uncultured bacterium clone in the Bacteroidetes class (99% similarity). Various other Geobacter-like sequences were also enriched in most of the anode biofilms. While the anode communities in replicate reactors for each substrate generally converged to a reproducible community, there were some variations in the relative distribution of these putative anode-reducing Geobacter-like strains. Firmicutes were found only in glucose-fed MFCs, presumably serving the roles of converting complex carbon into simple molecules and scavenging oxygen. The maximum current density in these systems was negatively correlated with internal resistance variations among replicate reactors and, likely, was only minimally affected by anode community differences in these two-chamber MFCs with high internal resistance.