Investigating Electrosprayed Droplets Using Particle-into-Liquid Sampling for Nanoliter Electrochemical Reactions
Investigating Electrosprayed Droplets Using Particle-into-Liquid Sampling for Nanoliter Electrochemical Reactions
复制标题
使用颗粒进入液体采样研究纳升电化学反应的电喷雾液滴
DOI：
10.1021/jasms.2c00338
复制
发表时间：
2023
影响因子：
3.2
通讯作者：
Dick, Jeffrey E.
中科院分区：
文献类型：
--
作者：
Park, Nathaneal A.;Glish, Gary L.;Dick, Jeffrey E.
电喷雾电离(ESI)是一种强大的电离技术，可以从样品溶液中产生带电的溶剂液滴和裸露的分析物离子。尽管在质谱学中得到了广泛的应用，部分原因是由于电离过程中形成的离子的低内能，但关于雾化过程中液滴破裂和分子行为的确切动力学仍然存在一些未知，关于各种类型的分子如何在ESI过程中获得电荷的研究仍在进行中。在此之前，作者介绍了一种新的气溶胶测量技术，即纳升电化学反应的颗粒到液体采样(Pilsner)。目前的工作介绍了一种新的方法，利用Pilsner来检查ESI过程中产生的颗粒，使用商业上可用的恒电位器，使用简单的分析技术。这项技术被应用于检测电喷雾液滴上的电荷，包括使用荧光试剂估计单个ESI液滴上的电荷数量。这项技术为探索ESI过程中液滴产生和离子释放的复杂过程提供了另一种工具。
Electrospray ionization (ESI) is a powerful ionization technique that can generate charged solvent droplets and bare analyte ions from sample solutions. Despite seeing extensive use in mass spectrometry due in part to the low internal energy deposited into the ions formed during ionization, some unknowns persist regarding the exact dynamics of droplet breakup and molecule behavior during spray, and research is still underway regarding how various types of molecules acquire charge during the ESI process. Previously, the authors introduced a novel aerosol measurement technique, particle-into-liquid sampling for nanoliter electrochemical reactions (PILSNER). The current work introduces a new method utilizing PILSNER for the examination of the particles generated during ESI using simple analysis techniques with a commercially available potentiostat. This technique is applied in this work for the detection of charges on electrosprayed droplets, including the estimation of the number of charges on individual ESI droplets using a fluorescent proxy. This technique provides an additional tool for the exploration of the complex process of droplet generation and ion liberation during ESI.