Enzymatic cycling assay for phenylpyruvate.
Enzymatic cycling assay for phenylpyruvate.
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苯丙酮酸的酶循环测定。
DOI：
10.1016/0003-2697(89)90469-7
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发表时间：
1989
影响因子：
2.9
通讯作者：
Asano,Y
中科院分区：
文献类型：
--
作者：
Cooper,AJ;Leung,LK;Asano,Y
本文介绍了测定去蛋白组织提取物中l-苯丙氨酸和苯丙酮酸的酶循环法。试验1将谷氨酰胺转氨酶K与L-苯丙氨酸脱氢酶偶联。试验2结合了苯丙氨酸脱氢酶、l-氨基酸氧化酶和过氧化氢酶。在这两种测定中，酪氨酸和一些其他氨基酸（或其α-酮酸类似物）可以在很小程度上取代苯丙氨酸（或苯丙酮酸）。因此，如果要测量苯丙氨酸，必须对反应的非特异性进行校正。通过在阳离子交换柱上去除苯丙氨酸，可以测量组织提取物中的苯丙酮酸。正常大鼠肝、肾和脑中苯丙酮酸的浓度（μmol/kg）分别为2.1 ± 1.1（n = 8）、1.8 ± 0.4（n = 4）和3.3 ± 0.6（n = 4）。
Enzymatic cycling assays for the determination of l-phenylalanine and phenylpyruvate in deproteinized tissue extracts are described. Assay 1 couples glutamine transaminase K with l-phenylalanine dehydrogenase. Assay 2 combines phenylalanine dehydrogenase, l-amino acid oxidase, and catalase. In both assays, tyrosine and some other amino acids (or their α-keto acid analogs) can replace phenylalanine (or phenylpyruvate) to a small extent. Thus, if phenylalanine is to be measured a correction must be made for the nonspecificity of the reaction. By removing phenylalanine on a cation-exchange column it was possible to measure phenylpyruvate in tissue extracts. Concentrations of phenylpyruvate (μmol/kg) in normal rat liver, kidney, and brain were 2.1 ± 1.1 (n = 8), 1.8 ± 0.4 (n = 4), and 3.3 ± 0.6 (n = 4), respectively.