REGULATION OF RNA-POLYMERASE-SIGMA SUBUNIT SYNTHESIS IN ESCHERICHIA-COLI - INTRACELLULAR LEVELS OF SIGMA(70) AND SIGMA(38)
REGULATION OF RNA-POLYMERASE-SIGMA SUBUNIT SYNTHESIS IN ESCHERICHIA-COLI - INTRACELLULAR LEVELS OF SIGMA(70) AND SIGMA(38)
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DOI：
10.1128/jb.177.23.6832-6835.1995
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发表时间：
1995-12-01
影响因子：
3.2
通讯作者：
ISHIHAMA, A
中科院分区：
文献类型：
--
作者：
JISHAGE, M;ISHIHAMA, A
Western免疫印迹法测定了大肠杆菌MC4100细胞内两个主要的sigma亚基sigma(70) （rpoD基因产物sigma(D)）和酸sigma(38) （rpoS基因产物sigma(S)）的表达水平，结果表明，从指数生长期到固定期，sigma（70）的表达水平维持在50 ~ 80 fmol / μ g。而sigma（38）蛋白水平在指数生长期低于检测水平，但在细胞停止生长进入固定期时上升到sigma（70）水平的30%，除固定期外，sigma（38）水平在指数期热休克和固定期渗透休克两种情况下均有升高。
The intracellular levels of two principal sigma subunits, sigma(70) (sigma(D), the rpoD gene product) acid sigma(38) (sigma(S), the rpoS gene product), in Escherichia coli MC4100 were determined by a quantitative Western immunoblot analysis, Results indicate that the level of sigma(70) is maintained at 50 to 80 fmol per mu g of total proteins throughout the transition from the exponential growth phase to the stationary phase, while the level of sigma(38) protein is below the detection level at the exponential growth phase but increases to 30% of the level of sigma(70) when cell growth stops to enter into the stationary phase, Beside the stationary phase, the increase in sigma(38) level was observed in two cases: exposure to heat shock at the exponential phase and osmotic shock at the stationary phase.