Rice OGR1 encodes a pentatricopeptide repeat-DYW protein and is essential for RNA editing in mitochondria
Rice OGR1 encodes a pentatricopeptide repeat-DYW protein and is essential for RNA editing in mitochondria
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DOI：
10.1111/j.1365-313x.2009.03909.x
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发表时间：
2009-09-01
期刊：
影响因子：
7.2
通讯作者：
An, Gynheung
中科院分区：
文献类型：
--
作者：
Kim, Sung-Ryul;Yang, Jung-Il;An, Gynheung
RNA编辑是通过核苷酸的插入、缺失和转换来改变RNA序列。在开花植物中，从细胞器基因组转录的RNA的特定胞苷残基被转化为尿苷。在高等植物的叶绿体中存在大约35个编辑位点;在拟南芥中已经鉴定了六个参与RNA编辑的五肽重复基因。然而，尽管在开花植物的线粒体RNA中发现了大约500个编辑位点，但据报道，在拟南芥中只有一个基因参与这种编辑。在这里，我们确定了水稻突变体，这些突变体在五个线粒体转录本上的七个特定RNA编辑位点上有缺陷。它们的表型多样，包括种子发芽延迟、生长迟缓、矮化和不育。来自杂合植物的突变种子是不透明的。这种突变，命名为不透明和生长迟缓1（ogr 1），是由T-DNA插入到编码含有DYW基序的五肽重复蛋白的基因中产生的。OGR 1-sGFP融合蛋白定位于线粒体。突变体中OGR 1的异位表达补充了改变的表型。我们的结论是，OGR 1是水稻线粒体中RNA编辑所必需的，并且是正常生长和发育所必需的。
RNA editing is the alteration of RNA sequences via insertion, deletion and conversion of nucleotides. In flowering plants, specific cytidine residues of RNA transcribed from organellar genomes are converted into uridines. Approximately 35 editing sites are present in the chloroplasts of higher plants; six pentatricopeptide repeat genes involved in RNA editing have been identified in Arabidopsis. However, although approximately 500 editing sites are found in mitochondrial RNAs of flowering plants, only one gene in Arabidopsis has been reported to be involved in such editing. Here, we identified rice mutants that are defective in seven specific RNA editing sites on five mitochondrial transcripts. Their various phenotypes include delayed seed germination, retarded growth, dwarfism and sterility. Mutant seeds from heterozygous plants are opaque. This mutation, named opaque and growth retardation 1 (ogr1), was generated by T-DNA insertion into a gene that encodes a pentatricopeptide repeat protein containing the DYW motif. The OGR1-sGFP fusion protein is localized to mitochondria. Ectopic expression of OGR1 in the mutant complements the altered phenotypes. We conclude that OGR1 is essential for RNA editing in rice mitochondria and is required for normal growth and development.