The complete mitochondrial genome of the broad-winged damselfly Mnais costalis Selys (Odonata: Calopterygidae) obtained by next-generation sequencing
The complete mitochondrial genome of the broad-winged damselfly Mnais costalis Selys (Odonata: Calopterygidae) obtained by next-generation sequencing
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DOI：
10.1080/13887890.2016.1234980
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发表时间：
2016-01-01
影响因子：
0.9
通讯作者：
Watts, Phillip C.
中科院分区：
文献类型：
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作者：
Lorenzo-Carballa, M. Olalla;Tsubaki, Yoshitaka;Watts, Phillip C.
我们使用下一代测序来表征豆娘Mnais costalis的完整线粒体基因组。Illumina配对末端读段与COI和16S序列比对，然后使用迭代从头映射程序进行扩展。最终组装成一个15,487 bp的连续序列，包含所有标准线粒体编码区和假定的a +T富区。costalis有丝分裂基因组的基因结构与其他齿形动物相似，包括13个蛋白质编码基因、大小rRNA基因和22个tRNA基因。我们发现三个基因间间隔物也存在于所有可用的完整有丝分裂基因组中。costalis有丝分裂基因组的碱基组成分别为40% (A)、20% (C)、14% (G)和26% (T)，其中A+T含量较高（66%）。齿形鼠线粒体全基因组的描述为目前可用的齿形鼠线粒体基因组增加了一份，并将有助于改进引物设计，用于未来的群体遗传研究。一项系统发育分析包括目前可获得的odonates线粒体基因组序列表明，Epiophlebia superstes与颧翅目的关系比与异翅目的关系更密切。
We used next-generation sequencing to characterise the complete mitochondrial genome of the damselfly Mnais costalis (Odonata, Calopterygidae). Illumina paired end reads were mapped against COI and 16S sequences from M. costalis and then extended using an iterative de novo map procedure. The final assembly was a contiguous sequence of 15,487 bp, which contained all standard mitochondrial coding regions and the putative A+T rich region. The gene configuration of the M. costalis mitogenome is similar to that of other odonates, comprising 13 protein-coding genes, large and small rRNA genes, and 22 tRNA genes. We found three intergenic spacers that are also present in all available whole odonate mitogenomes. Base composition of the M. costalis mitogenome is 40% (A), 20% (C), 14% (G) and 26% (T), with a high A+T content (66%). The characterisation of the complete mitochondrial genome of M. costalis adds to the growing list of mitogenomes currently available for odonates, and will help to improve primer design for future population genetic studies. A phylogenetic analysis including the currently available mitochondrial genome sequences of odonates suggests that Epiophlebia superstes is more closely related to the Zygoptera than to the Anisoptera.