SYNAPTIC AND NONSYNAPTIC CHOLINERGIC INNERVATION OF THE VARIOUS TYPES OF NEURONS IN THE MAIN OLFACTORY-BULB OF ADULT-RAT - IMMUNOCYTOCHEMISTRY OF CHOLINE-ACETYLTRANSFERASE
SYNAPTIC AND NONSYNAPTIC CHOLINERGIC INNERVATION OF THE VARIOUS TYPES OF NEURONS IN THE MAIN OLFACTORY-BULB OF ADULT-RAT - IMMUNOCYTOCHEMISTRY OF CHOLINE-ACETYLTRANSFERASE
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DOI：
10.1016/0306-4522(95)00031-d
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发表时间：
1995-08-01
期刊：
影响因子：
3.3
通讯作者：
WOLFF, JR
中科院分区：
文献类型：
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作者：
KASA, P;HLAVATI, I;WOLFF, JR
用胆碱乙酰转移酶免疫细胞化学方法，对成年大鼠主嗅球胆碱能神经元的结构及其突触联系进行了分析。在肾小球内，胆碱能神经纤维被限制到股细分成小隔间，其内部含有感觉轴突，但没有胆碱能轴突的神经节。在所有层中检测到少量的胆碱乙酰转移酶神经元。超微结构分析显示选择性。在肾小球内区域和外网状层中与不同神经元类的三元突触关系。这些三联体由（i）胆碱能轴突，（ii）一个或多个肾小球周围或颗粒细胞树突，和（iii）通常一个中继细胞树突组成。在这些三联体中，不对称的胆碱能突触选择性地集中在肾小球周围或颗粒细胞的树突（芽和棘）上。在肾小球内，二尖瓣和簇状细胞树突紧密贴壁的一些胆碱能轴突静脉曲张，最丰富的近arborizations的顶端树突。然而，胆碱能突触从来没有看到任何中继细胞树突。在外网状层，胆碱能突触存在于浅层短轴突细胞的所有部分。在内网状层和颗粒细胞层，胆碱能轴突与颗粒细胞芽呈紧密并置或不对称突触，提示胆碱能投射主要集中在中间神经元上。在中继细胞上，直接的乙酰胆碱效应会发生，但这些效应必须基于树突表面的非突触乙酰胆碱释放。
The cholinergic neuronal structures and their synaptic connections in the main olfactory bulb of adult rats were analysed by using choline acetyltransferase immunocytochemistry. Within the glomeruli, cholinergic nerve fibers were restricted to strands which subdivided the neuropil into small compartments, the interior of which contained sensory axons but was devoid of cholinergic axons. Small numbers of choline acetyltransferase neurons were detected in all layers. Ultrastructural analysis revealed selective. triadic synaptic relationships with different neuron classes in the intraglomerular area and in the external plexiform layer. These triads were made up of (i) a cholinergic axon, (ii) one or several periglomerular or granule cell dendrites, and (iii) usually one relay cell dendrite. In these triads, asymmetric cholinergic synapses were selectively focused on dendrites (gemmules and spines) of periglomerular or granule cells. Within the glomerulus, mitral and tufted cell dendrites were closely apposed to some cholinergic axon varicosities, most abundantly near arborizations of the apical dendrites. However, cholinergic synapses were never seen on any relay cell dendrite. In the external plexiform layer, cholinergic synapses were present on all parts of the superficial short-axon cells. In the internal plexiform layer and the granule cell layer, cholinergic axon varicosities exhibited close apposition or asymmetric synapses with granule cell gemmules.The data suggest that cholinergic projections from the basal forebrain to the main olfactory bulb focus synaptic innervation on interneurons. On relay cells, direct acetylcholine effects mat occur, but these must be based on non-synaptic acetylcholine release at the surface of their dendrites.