Angiotensin II type 2 receptor is expressed in human sperm cells and is involved in sperm motility
Angiotensin II type 2 receptor is expressed in human sperm cells and is involved in sperm motility
复制标题
DOI：
10.1016/j.fertnstert.2015.11.004
复制
发表时间：
2016-03-01
影响因子：
6.7
通讯作者：
Irazusta, Jon
中科院分区：
文献类型：
--
作者：
Gianzo, Marta;Munoa-Hoyos, Iraia;Irazusta, Jon
目的：为了探讨人类精子中血管紧张素II 2型受体（AT 2 R）的存在及其在精子生育力status.Design的含义：我们进行了AT 2 R的表达测定，逆转录聚合酶链反应，Western blot，免疫荧光，流式细胞术技术在人类精子细胞。AT 2 R阳性精子细胞的百分比通过流式细胞术进行分析。设置：辅助生殖单位和学术研究实验室。患者：来自毕尔巴鄂IVI诊所的97份人类精液样本。干预措施：根据世界卫生组织指南对所有样本进行检查和分类。采用不连续硅胶梯度（45%-90%）和上游技术（swim-up techniques）从精液中分离精子。主要结果测量：精子中AT 2 R的存在和位置以及流式细胞术测量AT 2 R阳性精子细胞的百分比。免疫荧光研究表明，AT 2 R主要定位于精子头部的赤道段。AT 2 R水平与精子运动参数相关。特别是，我们发现了一个显着的正相关关系AT 2 R和精子的前向运动等级和一个显着的负相关关系与不动的精子，无论是在新鲜的精液样本和制备的精子细胞。关于病理研究，AT 2 R的水平测定流式细胞仪在弱精子症男性精子低于normozoospermic controls. Conclusions（S）：血管紧张素Ⅱ 2型受体存在于人类精液中，并可能参与控制精子活力。深入了解与精子运动有关的蛋白质有助于阐明这些蛋白质在男性不育中的作用，并建立新的男性不育生物标志物。(C)2016年，美国生殖医学会（American Society for Reproductive Medicine）
Objective: To investigate the presence of angiotensin II type 2 receptor (AT2R) in human spermatozoa and its implication in sperm fertility status.Design: We carried out expression assays for AT2R by reverse transcription-polymerase chain reaction, Western blot, immunofluorescence, and flow cytometry techniques in human sperm cells. Percentage of AT2R-positive sperm cells was analyzed by flow cytometry.Setting: Assisted reproduction unit and academic research laboratory.Patient(s): Ninety-seven human semen samples from the Clinica IVI Bilbao.Intervention(s): All samples were examined and classified according to World Health Organization guidelines. Spermatozoa were isolated from semen on discontinuous colloidal silica gradient (45%-90%) and swim-up techniques.Main Outcome Measure(s): Presence and location of the AT2R in spermatozoa and percentage of AT2R-positive sperm cells measured by flow cytometry.Result(s): We demonstrated the existence of AT2R and its transcript in human sperm by Western blot and reverse transcription-polymerase chain reaction. Immunofluorescence studies showed that AT2R is mainly located at the equatorial segment of the sperm head. The AT2R levels were associated with sperm motility parameters. Particularly, we found a significant positive correlation between AT2R and spermatozoa with progressive motility grade and a significant negative correlation with immotile spermatozoa, both in fresh semen samples and in prepared sperm cells. Regarding pathologic studies, the levels of AT2R measured by flow cytometry were lower in spermatozoa of asthenozoospermic men than in normozoospermic controls.Conclusion(s): Angiotensin II type 2 receptor is present in human semen and may be involved in the control of sperm motility. In-depth understanding of the proteins involved in sperm motility can help to elucidate the role of these proteins in male infertility as well as to establish new biomarkers for male infertility. (C) 2016 by American Society for Reproductive Medicine.