A RECQ5-RNA polymerase II association identified by targeted proteomic analysis of human chromatin
A RECQ5-RNA polymerase II association identified by targeted proteomic analysis of human chromatin
复制标题
DOI：
10.1073/pnas.0804424105
复制
发表时间：
2008-06-24
影响因子：
11.1
通讯作者：
Liu, Yilun
中科院分区：
文献类型：
--
作者：
Aygun, Ozan;Svejstrup, Jesper;Liu, Yilun
尽管参与DNA相关过程（如DNA复制、修复和转录）的活性因子形式与染色质相关，但很少从染色质中纯化蛋白质。在这里，我们描述了一种分离染色质相关因子的方案，并用它来鉴定与人RNA聚合酶II （RNAPII）相互作用的蛋白质。我们的数据证实了RECQ5解旋酶是一个真正的rnapii相关蛋白。RECQ5-RNAPII相互作用是直接的，由RNAPII的RPB1亚基介导，RECQ5似乎是人类RECQ解旋酶家族中唯一与RNAPII相关的成员。这些数据表明，RECQ5解旋酶在转录和基因组稳定性的界面上具有意想不到的作用。
Although the active forms of factors involved in DNA-related processes such as DNA replication, repair, and transcription are associated with chromatin, proteins are rarely purified from this source. Here, we describe a protocol for the isolation of chromatin-associated factors and use it to identify proteins interacting with human RNA polymerase II (RNAPII). Our data establish RECQ5 helicase as a bona fide RNAPII-associated protein. The RECQ5-RNAPII interaction is direct and is mediated by the RPB1 subunit of RNAPII, and RECQ5 appears to be the only member of the human RECQ family of helicases that associates with RNAPII These data suggest an unexpected role for RECQ5 helicase at the interface of transcription and genomic stability.