Human hepatic stellate cell line (LX-2) exhibits characteristics of bone marrow-derived mesenchymal stem cells
Human hepatic stellate cell line (LX-2) exhibits characteristics of bone marrow-derived mesenchymal stem cells
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DOI：
10.1016/j.yexmp.2011.09.002
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发表时间：
2011-12-01
影响因子：
3.6
通讯作者：
Covas, Dimas Tadeu
中科院分区：
文献类型：
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作者：
Castilho-Fernandes, Andrielle;de Almeida, Danilo Candido;Covas, Dimas Tadeu
LX-2细胞系具有肝星状细胞（hsc）的特征，被认为是肝微循环系统的周细胞。最近的研究表明造血干细胞可能起源于间充质细胞。我们对LX-2细胞进行了广泛的表征，并将其特征与间充质细胞的特征进行了比较。我们的数据显示LX-2细胞具有类似于活化的造血干细胞以及骨髓来源的间充质干细胞（BM-MSCs）的表型。我们的免疫表型分析显示LX-2细胞对活化的HSC标志物（α SMA、GFAP、nestin和CD271）和经典的间充质制造物（CD105、CD44、CD29、CD13、CD90、HLA - i类、CD73、CD49e、CD166和CD146）呈阳性，但对内皮标记物CD31和内皮祖细胞标记物CD133以及造血标记物（CD45和CD34）呈阴性。LX-2细胞也表达在永生化和原代BM-MSCs中发现的相同转录物（vimentin）。膜联蛋白5，胶原蛋白1A。NG2和CD140b)，尽管水平不同。我们发现LX-2细胞能够在体外分化成多系间充质细胞，并能在体内刺激新血管的形成。LX-2细胞似乎不具有致瘤潜能。因此，LX-2细胞系表现为与BM-MSCs相似的多能细胞系。这条线应该有助于进一步研究阐明肝脏再生机制，并为发展肝细胞为基础的治疗奠定基础。(C) 2011爱思唯尔公司版权所有。
The LX-2 cell line has characteristics of hepatic stellate cells (HSCs), which are considered pericytes of the hepatic microcirculatory system. Recent studies have suggested that HSCs might have mesenchymal origin. We have performed an extensive characterization of the LX-2 cells and have compared their features with those of mesenchymal cells. Our data show that LX-2 cells have a phenotype resembling activated HSCs as well as bone marrow-derived mesenchymal stem cells (BM-MSCs). Our immunophenotypic analysis showed that LX-2 cells are positive for activated HSC markers (alpha SMA, GFAP, nestin and CD271) and classical mesenchymal makers (CD105, CD44, CD29, CD13, CD90, HLA class-I, CD73, CD49e, CD166 and CD146) but negative for the endothelial marker CD31 and endothelial progenitor cell marker CD133 as well as hematopoietic markers (CD45 and CD34). LX-2 cells also express the same transcripts found in immortalized and primary BM-MSCs (vimentin. annexin 5, collagen 1A. NG2 and CD140b), although at different levels. We show that LX-2 cells are capable to differentiate into multilineage mesenchymal cells in vitro and can stimulate new blood vessel formation in vivo. LX-2 cells appear not to possess tumorigenic potential. Thus, the LX-2 cell line behaves as a multipotent cell line with similarity to BM-MSCs. This line should be useful for further studies to elucidate liver regeneration mechanisms and be the foundation for development of hepatic cell-based therapies. (C) 2011 Elsevier Inc. All rights reserved.