Identification of a major QTL for silique length and seed weight in oilseed rape (Brassicanapus L.)
Identification of a major QTL for silique length and seed weight in oilseed rape (Brassicanapus L.)
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DOI：
10.1007/s00122-012-1833-7
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发表时间：
2012-03
影响因子：
5.4
通讯作者：
Pu Yang;Chang Shu;Lin Chen;Jinsong Xu;Jiangsheng Wu;Kede Liu
中科院分区：
文献类型：
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作者：
Pu Yang;Chang Shu;Lin Chen;Jinsong Xu;Jiangsheng Wu;Kede Liu
角果长和粒重是甘蓝型油菜两个重要的产量相关性状，受数量性状基因座（QTL）控制。这两个特征背后的遗传基础目前在很大程度上是未知的。在这项研究中，我们进行了QTL分析SL和SW使用186重组自交系（RILs）来自S1，EMS突变体与极长角果和大的种子，和S2，自交系与定期角果长度和种子大小之间的杂交。在2008/09（SS09）和2009/10（SS10）生长季节在武汉种植RILs，并调查每个品系的平均SL和SW。10个非冗余QTL被确定为SL。其中，一个主要的QTL，cqSLA9，始终解释高达53.4%的SL的变化在不同环境中。其他的是次要的QTL和单独解释不到10%的SL的变化。在SS09和SS10环境下，共检测到9个非冗余QTL，其中主效QTL cqSWA9可解释28.2%的SW变异。另外，SL检测到3个加性互作效应，但互作效应较小，SW未检测到互作效应。该位点上的S1等位基因使SL和SW均增加，表明uqA9对SL和SW都具有多效性效应。在S1与No2127（一个重新合成的具有规则角果长度和种子大小的DH系）杂交的遗传上不同的RIL中证实了fuqA9的存在和作用。cqSLA 9杂合株系中的个体角果长度存在显著差异。研究结果表明，突变体S1的角果长度主要受qSLA9位点控制，这将为油菜高产育种的精细定位和分子标记辅助选择提供依据。
Silique length (SL) and seed weight (SW) are two important yield-related traits controlled by quantitative trait loci (QTL) in oilseed rape (Brassica napusL.). The genetic bases underlying these two traits are largely unknown at present. In this study, we conducted QTL analyses for SL and SW using 186 recombinant inbred lines (RILs) derived from a cross between S1, an EMS mutant with extremely long siliques and large seeds, and S2, an inbred line with regular silique length and seed size. RILs were grown in Wuhan in the 2008/09 (SS09) and 2009/10 (SS10) growing seasons, and mean SL and SW for each line were investigated. Ten non-redundant QTL were identified for SL. Of these, a major QTL,cqSLA9, consistently explained as much as 53.4% of SL variation across environments. The others are minor QTL and individually explained less than 10% of the SL variation. Nine non-redundant QTL were identified for SW. Of which, one major QTL,cqSWA9, explained as much as 28.2% of the total SW variation in the SS09 and SS10 environments. In addition, three additive by additive interactions with small effects were detected for SL, and no interactions were detected for SW. Interestingly, the two major QTL,cqSLA9for SL andcqSWA9for SW colocalized in the same chromosomal region and were integrated into a unique QTL,uqA9. The S1 allele at this locus increases both SL and SW, suggesting thatuqA9has pleiotropic effects on both SL and SW. The existence and effect ofuqA9was confirmed in genetically different RILs derived from the cross between S1 and No2127, a resynthesized DH line having regular silique length and seed size. Individuals in one residual heterozygous line forcqSLA9showed significant difference in silique length. The results in this study revealed that silique length in the S1 mutant is mainly controlled by thecqSLA9locus, which will be suitable for fine mapping and marker-assisted selection in rapeseed breeding for high yield.