An RNA-Binding Protein Secreted by a Bacterial Pathogen Modulates RIG-I Signaling
An RNA-Binding Protein Secreted by a Bacterial Pathogen Modulates RIG-I Signaling
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DOI：
10.1016/j.chom.2019.10.004
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发表时间：
2019-12-11
影响因子：
30.3
通讯作者：
Cossart, Pascale
中科院分区：
文献类型：
--
作者：
Pagliuso, Alessandro;To Nam Tham;Cossart, Pascale
RNA结合蛋白（RBP）通过控制结合RNA的功能来执行关键的细胞活动。广泛持有的假设，严格的细胞内的RBPs已受到挑战的发现分泌的RBPs。然而，胞外RBP已在真核生物中描述，而分泌的细菌RBP尚未报道。在这里，我们表明，细菌病原体单核细胞增生李斯特菌分泌一个小的RBP，我们命名为玉米。我们表明，玉米绑定L的一个子集。单核细胞增生RNA，导致其在细胞外介质中的积累。此外，在L.在单核细胞增多症感染中，玉米结合RIG-I，非自身RNA先天免疫传感器，增强干扰素β的产生。小鼠感染研究表明，玉米影响L。单核细胞增生毒力总之，我们的研究结果揭示了细菌RNA可以与RBP一起存在于细胞外，作为“社会RNA”在感染期间触发宿主反应。
RNA-binding proteins (RBPs) perform key cellular activities by controlling the function of bound RNAs. The widely held assumption that RBPs are strictly intracellular has been challenged by the discovery of secreted RBPs. However, extracellular RBPs have been described in eukaryotes, while secreted bacterial RBPs have not been reported. Here, we show that the bacterial pathogen Listeria monocytogenes secretes a small RBP that we named Zea. We show that Zea binds a subset of L. monocytogenes RNAs, causing their accumulation in the extracellular medium. Furthermore, during L. monocytogenes infection, Zea binds RIG-I, the non-self-RNA innate immunity sensor, potentiating interferon-beta production. Mouse infection studies reveal that Zea affects L. monocytogenes virulence. Together, our results unveil that bacterial RNAs can be present extracellularly in association with RBPs, acting as "social RNAs" to trigger a host response during infection.