Novel non-suppressing mutants of Escherichia coli tRNATyr su+3.
Novel non-suppressing mutants of Escherichia coli tRNATyr su+3.
复制标题
大肠杆菌 tRNATyr su 3 的新型非抑制突变体。
DOI：
10.1093/nar/13.7.2583
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发表时间：
1985
影响因子：
14.9
通讯作者：
Altman,S
中科院分区：
文献类型：
--
作者：
Furdon,P;Altman,S
在与成熟tRNA的二氢尿嘧啶环相对应的大肠杆菌tRNatyr基因区域，构建了几个添加和缺失突变。与亲本基因相比，这些突变体都没有可检测到的抑制功能，但一些突变体指导了成熟tRNA的合成。这些后一种突变体可能会影响tRNA被氨基酰化或与核糖体上的翻译机制相互作用的能力。
Several addition and deletion mutations were constructed in the region of the gene for Escherichia coli tRNATyrcorresponding to the dihydrouracil loop of the mature tRNA. None of these resulting mutants had detectable suppressor function compared to the parent gene yet some directed the synthesis of mature tRNA. These latter mutants may affect the ability of the tRNA to be aminoacylated or to interact with the translational machinery on the ribosome.