Decreased airway mucosal prostaglandin E2 production during airway obstruction in an animal model of asthma.
Decreased airway mucosal prostaglandin E2 production during airway obstruction in an animal model of asthma.
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哮喘动物模型气道阻塞期间气道粘膜前列腺素 E2 的产生减少。
DOI：
10.1164/ajrccm/146.3.586
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
M. Peters
中科院分区：
文献类型：
--
作者：
P. Gray;F. Derksen;R. Broadstone;N. E. Robinson;M. Peters
Heaves是马和矮种马的呼吸系统疾病，其特征是急性气道阻塞和气道高反应性发作。我们测量了前列腺素E2（PGE 2）和15-羟基二十碳四烯酸（15-HETE）的生产在体外气管上皮细胞从6个受影响的马在急性气道阻塞的时间相比，6个匹配的对照马。制备上皮和上皮下组织条，并用A23187、组胺和缓激肽刺激。通过放射免疫测定法定量来自条的培养基中的PGE 2和15-HETE。15-仅在两只主要动物的上皮条中发现HETE高于准确检测限，而在对照马中均未发现HETE，并且刺激条时15-HETE的量未增加。从受影响的马的上皮条往往产生较少的PGE 2比从对照组的马，有一个显着的相关性上皮PGE 2的生产和受影响的动物所需的时间，以发展气道阻塞。上皮下组织条从控制马产生显着更多的PGE 2在响应A23187和缓激肽比受影响的马条。我们的结论是，马气管上皮是不是一个显着的来源15-HETE。气喘马的气道粘膜PGE 2产生减少，这表明这种支气管舒张物质的相对减少可能是这种哮喘模型发病机制的一个因素。
Heaves is a respiratory disorder of horses and ponies characterized by bouts of acute airway obstruction and airway hyperresponsiveness. We measured prostaglandin E2 (PGE2) and 15-hydroxyeicosatetraenoic acid (15-HETE) production in vitro in tracheal epithelium obtained from six affected horses at the time of acute airway obstruction as compared with six matched control horses. Strips of epithelium and subepithelial tissue were prepared and stimulated with A23187, histamine, and bradykinin. The PGE2 and 15-HETE in media from strips was quantitated by radioimmunoassay. 15-HETE above the limits of accurate detection was found in epithelial strips of only two principal animals and in none of the control horses, and the amount of 15-HETE was not increased when strips were stimulated. Epithelial strips from affected horses tended to produce less PGE2 than did strips from control horses, and there was a significant correlation between epithelial PGE2 production and the time taken for affected animals to develop airway obstruction. Subepithelial tissue strips from control horses produced significantly more PGE2 in response to A23187 and bradykinin than did strips from affected horses. We conclude that equine tracheal epithelium is not a significant source of 15-HETE. Airway mucosal PGE2 production is reduced in horses with heaves, which suggests that a relative decrease in this bronchorelaxant substance may be a factor in the pathogenesis of this model of asthma.
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影响因子：
4.4
作者：
S. Kunkel;S. Chensue;S. Phan
通讯作者：
S. Kunkel;S. Chensue;S. Phan
环氧合酶产品在小马急性气道阻塞和气道高反应性中的作用。
DOI：
10.1164/ajrccm/140.1.154
发表时间：
1989
期刊：
The American review of respiratory disease
影响因子：
--
作者：
Gray,PR;Derksen,FJ;Robinson,NE;Carpenter-Deyo,LJ;Johnson,HG;Roth,RA
通讯作者：
Roth,RA
花生四烯酸 15-脂氧合酶细胞表达的异质性：对生物活性的影响。
DOI：
10.1016/0005-2760(89)90257-9
发表时间：
1989
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Holtzman,MJ;Pentland,A;Baenziger,NL;Hansbrough,JR
通讯作者：
Hansbrough,JR
15-羟基二十碳四烯酸的新作用。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Vanderhoek,JY;Tare,NS;Bailey,JM;Goldstein,AL;Pluznik,DH
通讯作者：
Pluznik,DH
环氧合酶抑制剂可增加犬气管肌肉对副交感神经原位刺激的反应。
DOI：
10.1152/jappl.1990.68.6.2597
发表时间：
1990
期刊：
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子：
--
作者：
Bethel,RA;McClure,CL
通讯作者：
McClure,CL