Interplay between Carotenoids, Abscisic Acid and Jasmonate Guides the Compatible Rice-Meloidogyne graminicola Interaction.
Interplay between Carotenoids, Abscisic Acid and Jasmonate Guides the Compatible Rice-Meloidogyne graminicola Interaction.
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DOI：
10.3389/fpls.2017.00951
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发表时间：
2017
影响因子：
5.6
通讯作者：
Gheysen G
中科院分区：
文献类型：
--
作者：
Kyndt T;Nahar K;Haeck A;Verbeek R;Demeestere K;Gheysen G
在这项研究中，我们的特点是类胡萝卜素和叶绿素的作用，在定居的根结线虫（RKN）的禾本科根结线虫和单子叶模式植物水稻（水稻）之间的兼容性相互作用。先前的转录组数据显示，类胡萝卜素和叶绿素生物合成基因在线虫诱导的巨细胞和胆囊组织中的差异表达。代谢物测定表明，虫瘿确实积累叶绿素a，B和类胡萝卜素，以及激素脱落酸（阿坝）。当外源阿坝作用于水稻植株或ABA生物合成被抑制时，发现虫瘿形成和线虫发育显著增加，表明阿坝在这种相互作用中的复杂作用。阿坝应用抑制茉莉酸（JA）在植物中的水平，而ABA的生物合成抑制导致JA水平增加证实阿坝和JA在水稻根之间的拮抗作用。此外，阿坝和JA的联合应用表明ABA效应可以克服JA诱导的防御。基于这些观察，我们假设叶绿素和类胡萝卜素前体的积累将有利于线虫感染。事实上，当在不同步骤化学阻断类胡萝卜素生物合成途径时，这导致植物中类胡萝卜素和叶绿素的差异积累，检测到类胡萝卜素和叶绿素的积累与水稻对RKN的易感性之间的积极和明确的联系。
In this study, we have characterized the role of carotenoids and chlorophyll in the compatible interaction between the sedentary root knot nematode (RKN) Meloidogyne graminicola and the monocot model plant rice (Oryza sativa). Previous transcriptome data showed a differential expression of carotenoid and chlorophyll biosynthesis genes in nematode-induced giant cells and gall tissue. Metabolite measurement showed that galls indeed accumulate chlorophyll a, b and carotenoids, as well as the hormone abscisic acid (ABA). When ABA was externally applied on rice plants, or when ABA-biosynthesis was inhibited, a significant increase in gall formation and nematode development was found, showing the complex role of ABA in this interaction. ABA application suppressed jasmonic acid (JA) levels in the plants, while ABA-biosynthesis inhibition lead to increased JA levels confirming an antagonism between ABA and JA in rice roots. In addition, combined applications of ABA and JA showed that the ABA-effect can overcome JA-induced defense. Based on these observations, we hypothesized that the accumulation of chlorophyll and carotenoid precursors would be beneficial to nematode infection. Indeed, when chemically blocking the carotenoid biosynthesis pathway at different steps, which leads to differential accumulation of carotenoids and chlorophyll in the plants, a positive and clear link between accumulation of carotenoids and chlorophyll and rice susceptibility to RKN was detected.