Mutation of calcineurin subunit B M118 influences the activities of NF-AT and p53, but not calcineurin expression level
Mutation of calcineurin subunit B M118 influences the activities of NF-AT and p53, but not calcineurin expression level
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钙调磷酸酶 B 亚基 M118 突变影响 NF-AT 和 p53 的活性，但不影响钙调磷酸酶表达水平
DOI：
10.1016/j.bbrc.2011.08.126
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发表时间：
2011-09-30
影响因子：
3.1
通讯作者：
Wei, Qun
中科院分区：
文献类型：
--
作者：
Cheng, Jinbo;Tang, Wei;Wei, Qun
钙调磷酸酶（Calcineurin，CN）是一种钙/钙调素依赖性磷酸酶，由催化性A亚基（CnA）和调节性B亚基（CnB）组成。内源性CnA和CnB在癌细胞系中具有很强的相关性。通过将CnB及其突变体引入细胞，我们表明CnB不会增加CnA的表达，但会保护其免受降解。CnB M118是与CnA紧密结合所必需的。CnB M118的点突变也不增加CnA的表达，但保护其免于降解。此外，CnB M118 K不能增强CnA诱导的HeLa-s细胞中NF-AT和p53的活性。CnB M118的突变可能被证明是诊断一些重要疾病（如阿尔茨海默病）的有价值的标志物。皇冠版权所有（C）2011由爱思唯尔公司出版。All rights reserved.
Calcineurin (CN) is a Ca(2+)/calmodulin-dependent phosphatase, which consists of a catalytic A-subunit (CnA) and a regulatory B-subunit (CnB). Endogenous CnA and CnB have a strong corelationship in cancer cell lines. Through the introduction of CnB and its mutants in cells, we show that CnB does not increase the expression of CnA but protects it from degradation. CnB M118 is necessary for tight binding to CnA. Point mutations of CnB M118 also do not increase the expression of CnA but protect it from degradation. Furthermore, CnB M118K fails to enhance the activities of NF-AT and p53 induced by CnA in HeLa-s cells. Mutations in CnB M118 may prove to be a valuable marker in the diagnostics of some important illnesses such as Alzheimer's disease. Crown Copyright (C) 2011 Published by Elsevier Inc. All rights reserved.