Ultrastructural Pathology of Rat Lung Injury Induced by Ischemic Acute Kidney Injury
Ultrastructural Pathology of Rat Lung Injury Induced by Ischemic Acute Kidney Injury
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DOI：
10.3109/01913123.2013.833562
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发表时间：
2013-12-01
影响因子：
1
通讯作者：
Li, Xiaoming
中科院分区：
文献类型：
--
作者：
Zang, Dongyu;Shao, Youzhi;Li, Xiaoming
缺血性急性肾损伤（AKI）是住院患者常见的并发症，常诱发急性肺损伤（ALI）。许多研究都集中在AKI和ALI之间的相关性上，但AKI相关ALI的潜在机制至今仍不清楚。原因之一是aki相关ALI的超微结构病理证据很少，需要积累。本研究旨在观察缺血性AKI大鼠ALI的超微结构变化，揭示缺血性AKI大鼠ALI的白细胞转运。为此，本研究采用了光学显微镜（LM）和电子显微镜（EM）以及形态计量学分析。LM观察显示aki诱导的ALI有明显的肺泡塌陷、肺泡出血和间质水肿。EM检查显示，AKI肺的肺泡上皮细胞（包括I型和II型细胞）坏死，内皮细胞发生凋亡，间质细胞发生坏死。AKI大鼠肺泡II型细胞明显缩小，板层小体减少或消失。AKI肺的面积上白细胞数值密度（N-A）明显高于假肺。基于电镜检查和形态学分析的形态学标准，我们认为AKI引起的肺损伤涉及坏死，包括坏死下垂和细胞凋亡。炎症对AKI大鼠急性肺损伤也有促进作用。
Ischemic acute kidney injury (AKI) is a common complication during inpatient hospitalization, and often induces acute lung injury (ALI). A lot of studies have concentrated on the relevance between AKI and ALI, but the underlying mechanisms of AKI- associated ALI have remained unclear until now. One reason is that evidence of the ultrastructural pathology of AKI-associated ALI has been scarce and needed to be accumulated. The aims of present study are to observe ultrastructural changes, and to reveal leukocyte trafficking of ALI induced by ischemic AKI in rats. For this purpose light microscopy (LM) and electron microscopy (EM), as well as morphometric analysis, were employed in present study. LM observations revealed distinct regions of collapsed alveoli, hemorrhage in alveoli, and interstitial edema in AKI-induced ALI. EM examinations provided facts that alveolar epithelial cells, including type I and type II cells, were necrotic, and endothelia cells undergoing apoptosis as well as interstitial cells undergoing necroptosis were noted in AKI lungs. In addition, shrinkage and decreased or disappeared lamellar bodies were evident in alveolar type II cell of AKI rat lungs. Leukocyte numerical density on area (N-A) in AKI lungs was significantly more than that in sham lungs. Based on the morphological criteria from EM examinations and morphometric analysis, a conclusion was that necrosis, including necroptosis, and apoptosis were involved in damaged lung induced by AKI. And inflammation also contributed to acute lung injury of rats with AKI.