Hormonal regulation of the osteoblastic phenotype expression in neonatal murine calvarial cells
Hormonal regulation of the osteoblastic phenotype expression in neonatal murine calvarial cells
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DOI：
10.1007/s002239900624
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发表时间：
1999-04-01
影响因子：
4.2
通讯作者：
Fry, D
中科院分区：
文献类型：
--
作者：
Chen, TL;Fry, D
来自胎鼠颅骨的成骨细胞培养物为研究地塞米松（DEX）和1，25二羟维生素D-3 [1，25（OH）（2）D-3]对基因表达的影响提供了流行的模型，但来自小鼠颅骨细胞的数据很少。大鼠和小鼠成骨细胞对这些激素的种属特异性反应以前已有报道。在本研究中，我们研究了DEX和1，25（OH）（2）D-3对小鼠颅骨细胞成骨表型表达的影响。这些小鼠成骨细胞样（MOB）细胞表达碱性磷酸酶（ALP）活性和骨钙素，并形成钙化结节。与大鼠颅骨细胞不同，DEX抑制MOB中ALP活性和结节形成。1，25（OH）（2）D-3促进MOB合成骨钙素，DEX降低MOB合成骨钙素。1，25（OH）（2）D-3不影响结核的数量，但增加其大小。在培养开始时仅用DEX处理细胞2天增强了1，25（OH）（2）D-3对ALP的作用。我们发现，在小鼠颅骨细胞中，DEX抑制和1，25（OH）（2）D-3增强ALP活性，骨钙素合成和钙化结节形成。这与先前关于大鼠颅骨细胞的报道相反，在大鼠颅骨细胞中，DEX是阳性的，而1，25（OH）（2）D-3可以是成骨细胞表型的负调节剂。这些结果表明，在成骨细胞表型的调节中，小鼠和大鼠之间存在着深刻的种属特异性差异。
Osteoblastic cell cultures from fetal rat calvariae have provided a popular model for studying the effects of dexamethasone (DEX) and 1,25 dihydroxyvitamin D-3 [1,25(OH)(2)D-3] on gene expression but data from murine calvarial cells are scarce. Species-specific responses of rat and mouse osteoblastic cells to these hormones have been reported previously. In the present study, we investigated the effects of DEX and 1,25(OH)(2)D-3 on expression of the osteoblastic phenotype by mouse calvarial cells. These murine osteoblast-like (MOB) cells expressed alkaline phosphatase (ALP) activity and osteocalcin and formed calcified nodules. Unlike the rat calvarial cells, ALP activities and nodule formation in MOB were inhibited by DEX. 1,25(OH)(2)D-3 enhanced and DEX lowered the amount of osteocalcin synthesized by MOB. 1,25(OH)(2)D-3 did not affect the number of nodules, but increased their sizes. Treating the cells for 2 days with only DEX at the beginning of the culture enhanced the effect of 1,25(OH)(2)D-3 on ALP. We found that in murine calvarial cells, DEX inhibits and 1,25(OH)(2)D-3 enhances ALP activity, osteocalcin synthesis, and calcified nodule formation. This is in contrast to previous reports of rat calvarial cells where DEX is a positive and 1,25(OH)(2)D-3 can be a negative regulator of the osteoblastic phenotype. These results suggest that profound species-specific differences exist between mice and rats in the regulation of the osteoblastic phenotype.