The drosophila wnt, wingless, provides an essential signal for pre- and postsynaptic differentiation
The drosophila wnt, wingless, provides an essential signal for pre- and postsynaptic differentiation
复制标题
DOI：
10.1016/s0092-8674(02)01047-4
复制
发表时间：
2002-11-01
期刊：
影响因子：
64.5
通讯作者：
Budnik, V
中科院分区：
文献类型：
--
作者：
Packard, M;Koo, ES;Budnik, V
在脊椎动物神经肌肉接头(NMJ)中，AGRIN在突触发育中起着关键作用，但激活中央突触形成突触的分子在很大程度上是未知的。Wnt家族的成员被公认为是形态发生者，但最近也被认为与突触成熟有关。在这里，我们证明了果蝇Wnt，无翼(Wg)，是突触发育所必需的。我们发现Wg及其受体在谷氨酸能NMJ处表达，Wg由突触突触分泌。Wg的丢失导致靶依赖突触形成的急剧减少，新的突触要么不能形成活动区和突触后特化，要么这些都是惊人的异常。我们认为WG是突触前和突触后间隔协调发展的信号。
At vertebrate neuromuscular junctions (NMJs), Agrin plays pivotal roles in synapse development, but molecules that activate synapse formation at central synapses are largely unknown. Members of the Wnt family are well established as morphogens, yet recently they have also been implicated in synapse maturation. Here we demonstrate that the Drosophila Wnt, Wingless (Wg), is essential for synapse development. We show that Wg and its receptor are expressed at glutamatergic NMJs, and that Wg is secreted by synaptic boutons. Loss of Wg leads to dramatic reductions in target-dependent synapse formation, and new boutons either fail to develop active zones and postsynaptic specializations or these are strikingly aberrant. We suggest that Wg signals the coordinated development of pre- and postsynaptic compartments.