[Growth inhibition and mechanism of cetyltrimethyl ammonium chloride on Chlorella vulgaris].
[Growth inhibition and mechanism of cetyltrimethyl ammonium chloride on Chlorella vulgaris].
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发表时间：
2009-06
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通讯作者：
Yin Xu;Fei Ge;N. Tao;Run-liang Zhu;Na Wang
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作者：
Yin Xu;Fei Ge;N. Tao;Run-liang Zhu;Na Wang
在实验室分批培养条件下，研究了阳离子表面活性剂十六烷基三甲基氯化铵（CTAC）对小球藻的生长抑制作用。并通过测定藻类的吸附量、Zeta电位、酸性磷酸酶活性和超微结构等研究了其作用机理。结果表明，在0.1mg/L ~ 1 mg/L范围内，随着浓度的增加，CTAC的生长抑制作用增强，CTAC的96 h-EC 50为0.18mg/L。在0.3mg/L的CTAC存在下，培养8d，生物量的抑制率达到70.7%。当藻细胞Zeta电位从-12.5 mV增加到-6.7 mV时，氮和铁的吸收分别被抑制了83.9%和86.2%。酸性磷酸酶的相对活性下降到23.1%。细胞内可见胞浆肿胀、蛋白核变形、溶酶体肿胀。上述现象表明，CTAC提高了藻细胞的Zeta电位，从而抑制了氮和铁的吸收。此外，CTAC还可能影响藻类的磷代谢，改变藻细胞的超微结构。
Growth inhibition of cetyltrimethyl ammonium chloride (CTAC), a cationic surfactants, on Chlorella vulgaris was investigated at batch culture in laboratory. Furthermore, the corresponding mechanisms were studied by the determination of absorption capacity, Zeta potential, activity of acid phosphatase and ultrastructure of algae. Results show that the growth inhibition by CATC is enhanced with its concentration increasing from 0.1 mg/L to 1 mg/L, and 96 h-EC50 of CTAC is 0.18 mg/L. In the presence of 0.3 mg/L CTAC in 8 d, the inhibition efficiency of biomass reaches 70.7%. Meanwhile, the absorption of nitrogen and iron is inhibited 83.9% and 86.2% respectively with Zeta potential of algae cell increasing from -12.5 mV to -6.7 mV. Furthermore, the relative activity of acid phosphatase declines to 23.1% at the same time. Plasmolysis, distortion of pyrenoid and swelling of lysosome is observed in the cell. Above phenomena indicates that CTAC increases the Zeta potential of algae cell and thus inhibites the absorption of nitrogen and iron. In addition, CTAC may affect the metabolism of phosphorus and change the ultrastructure of algae cell.