Isolation of dieldrin- and endrin-degrading bacteria using 1,2-epoxycyclohexane as a structural analog of both compounds
Isolation of dieldrin- and endrin-degrading bacteria using 1,2-epoxycyclohexane as a structural analog of both compounds
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使用 1,2-环氧环己烷作为两种化合物的结构类似物分离狄氏剂和异狄氏剂降解细菌
DOI：
10.1007/s00253-008-1670-4
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发表时间：
2008
影响因子：
5
通讯作者：
H. Oyaizu
中科院分区：
文献类型：
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作者：
Emiko Matsumoto;Y. Kawanaka;Sun;H. Oyaizu
本报告描述了从土壤中选择性分离出对狄氏剂和异狄氏剂具有高降解活性的狄氏剂和异狄氏剂降解细菌。从土壤中的几个富集培养物安排与狄氏剂和异狄氏剂的几个结构类似物作为生长基质，并检查其对狄氏剂和异狄氏剂的降解活性。发现用1，2-环氧环己烷（ECH）富集培养物可好氧降解狄氏剂和异狄氏剂。变性梯度凝胶电泳（DGGE）结果表明，ECH富集培养物中有3种细菌占优势。在三种主要细菌中，有两种分离株，伯克霍尔德菌属。菌株MED-7和贪铜菌Cupriavidussp.菌株MED-5对狄氏剂和异狄氏剂具有较高的降解活性。MED-7和MED-5在ECH存在下降解14 d，对狄氏剂的降解率分别为49%和38%;对异狄氏剂的降解率分别为51%和40%。这些结果表明，ECH是一个有用的基板，选择性和有效的分离狄氏剂和异狄氏剂降解细菌含有大量的细菌的土壤。有趣的是，这两个菌株也可以降解狄氏剂和异狄氏剂，即使在无ECH。这些是第一批被证明在有氧条件下以狄氏剂和异狄氏剂作为唯一碳源和能源生长的微生物。
This report describes the selective isolation of dieldrin- and endrin-degrading bacteria from soil with high degradation activity toward dieldrin and endrin. Several enrichment cultures from the soil were arranged with several structural analogs of dieldrin and endrin as a growth substrate and examined for their degradation activities toward dieldrin and endrin. An enrichment culture with 1,2-epoxycyclohexane (ECH) was found to aerobically degrade dieldrin and endrin. Denaturing gradient gel electrophoresis (DGGE) indicated that three types of bacteria were predominant in the ECH enrichment culture. Of the three major bacteria, two isolates,Burkholderiasp. strain MED-7 andCupriavidussp. strain MED-5, showed high degradation activity toward dieldrin and endrin. The degradation efficiencies of strain MED-7 and MED-5 were 49% and 38% toward dieldrin, respectively, and 51% and 40% toward endrin, respectively, in the presence of ECH for 14 days. These results indicated that ECH was a useful substrate for selective and efficient isolation of dieldrin- and endrin-degrading bacteria from soil containing numerous bacteria. Interestingly, the two isolates could also degrade dieldrin and endrin even in the absence of ECH. These are the first microorganisms demonstrated to grow on dieldrin and endrin as the sole carbon and energy source under aerobic conditions.
未污染稻田土壤中微生物群对多氯联苯的厌氧生物降解
DOI：
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发表时间：
--
期刊：
World Journal of Microbiology and Biotechnology (印刷中)
影响因子：
--
作者：
Daisuke Baba;Tsuyoshi Yasuta;Naoko Yoshida;Yuko Kimura;Katsuhide Miyake;Yasushi Inoue;Koki Toyota;Arata Katayama
通讯作者：
Arata Katayama
影响因子：
4.4
作者：
MUYZER, G;DEWAAL, EC;UITTERLINDEN, AG
通讯作者：
UITTERLINDEN, AG