Types of nuclear endonuclease activity capable of inducing internucleosomal DNA fragmentation are completely different between human CD34+ cells and their granulocytic descendants.
Types of nuclear endonuclease activity capable of inducing internucleosomal DNA fragmentation are completely different between human CD34+ cells and their granulocytic descendants.
复制标题
人类 CD34 细胞及其粒细胞后代之间能够诱导核小体间 DNA 断裂的核内切酶活性类型完全不同。
作者：
N. Anzai;H. Kawabata;T. Hirama;H. Masutani;Y. Ueda;Y. Yoshida;M. Okuma
细胞凋亡的一个标志是由核酸内切酶的激活引起的核小体间DNA片段化。我们的特点是核酸内切酶活性的人髓细胞核切割自己的核染色质寡核小体长度的片段。正常人外周血多形核白细胞（PMNs）核内既有Ca ~（2+）/Mg ~（2+）依赖性酸性核酸内切酶活性，又有DNase Ⅱ样酸性核酸内切酶活性。不同粒细胞成熟阶段的正常骨髓未成熟髓系细胞具有相似的核酸酶活性。相反，在循环CD 34+细胞中显示出明显的差异，因为仅检测到Mg（2+）依赖性、Ca（2+）非依赖性内切核酸酶活性。与这些发现相一致的是，当CD 34+细胞在体外被诱导向粒细胞分化时，Ca 2 +/Mg（2+）依赖性和酸性核酸内切酶的出现伴随着Mg（2+）依赖性核酸内切酶的消失。所有谱系的白血病细胞系也具有Mg（2+）依赖性核酸酶活性。我们的研究结果表明，镁（2+）-依赖性核酸内切酶与造血祖细胞和核核酸酶在人髓系细胞中的相对活性的变化在粒细胞分化过程中显着协会。
A hallmark of apoptosis is internucleosomal DNA fragmentation resulting from the activation of endonucleases. We characterized the endonuclease activity of human myeloid cell nuclei that cleaved their own nuclear chromatin to oligonucleosomal length fragments. Polymorphonuclear leukocytes (PMNs) of normal peripheral blood contained both Ca2+/Mg(2+)-dependent and DNase II-like acidic endonuclease activities in their nuclei. Immature myeloid cells of normal bone marrow at various stages of granulocytic maturation had similar nuclease activities. In contrast, a clear difference was shown in the circulating CD34+ cells, in that only Mg(2+)-dependent, Ca(2+)-independent endonuclease activity was detected. Consistent with these findings is the emergence of the Ca2+/Mg(2+)-dependent and acidic endonuclease concomitantly with the disappearance of the Mg(2+)-dependent endonuclease when CD34+ cells were induced to differentiate in vitro toward granulocytes. Leukemic cell lines of all lineages also had Mg(2+)-dependent nuclease activity. Our results suggest an association of the Mg(2+)-dependent endonuclease with hematopoietic progenitor cells and that the relative activities of the nuclear nuclease in human myeloid cells change substantially during granulocytic differentiation.
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影响因子：
3.9
作者：
BARRY, MA;EASTMAN, A
通讯作者：
EASTMAN, A
影响因子：
11.2
作者：
S. Kaufmann
通讯作者：
S. Kaufmann
造血过程中的程序性细胞死亡（细胞凋亡）。
影响因子：
2.6
作者：
Koury,MJ
通讯作者：
Koury,MJ
影响因子：
56.9
作者：
KOURY, MJ;BONDURANT, MC
通讯作者：
BONDURANT, MC
来自人脾脏的 Ca2 /Mg(2 ) 依赖性核酸内切酶：纯化、特性和在细胞凋亡中的作用。
影响因子：
2.9
作者：
Ribeiro,JM;Carson,DA
通讯作者：
Carson,DA