In situ synthesis of multifunctional tellurium nanorods stabilized by polypeptide-engineered for photothermal-sonodynamic combination therapy of tumors
In situ synthesis of multifunctional tellurium nanorods stabilized by polypeptide-engineered for photothermal-sonodynamic combination therapy of tumors
复制标题
原位合成多肽工程稳定的多功能碲纳米棒，用于肿瘤的光热-声动力联合治疗
DOI：
10.1016/j.cej.2020.127989
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发表时间：
2021-05-12
影响因子：
15.1
通讯作者：
Liu, Bo
中科院分区：
文献类型：
--
作者：
Li, Chao-Qing;Zhao, Dong-Hui;Liu, Bo
含元素碲(Te)的纳米粒子在生物医学中的应用引起了人们的广泛关注。然而，理想的Te纳米粒子的合成方法和有效的抗癌策略仍然是一个巨大的挑战。在本研究中，原位合成了基因工程多肽(PC(10)ARGD)修饰的Te纳米棒(P-TeNRs)。透射电子显微镜证实，P-TeNRs具有良好的分散性，平均长度为25.2+/-4.65 nm，平均直径为6.5+/-0.83 nm。将PC(10)ARGD整合到Te纳米棒表面，提高了P-TeNRs的稳定性和生物相容性。P-TeNRs在近红外激光照射下表现出良好的光热转化性能，在超声(US)照射下具有产生活性氧(ROS)的能力，可以实现肿瘤的光热治疗和声动力学治疗。体内外实验结果表明，与单一光热疗法和声动力学疗法相比，联合疗法对乳腺4T1肿瘤具有协同作用。此外，体内毒性试验结果表明，合成的P-TeNRs的毒性可以忽略不计。这些结果表明，这种P-TeNRs可以被设计成一种新型的治疗肿瘤的光热-声动力学协同治疗的纳米平台。
Nanoparticles containing elemental tellurium (Te) have been aroused extensive attention in biomedical applications. However, the ideal synthesis methods and effective anti-cancer strategies of Te nanoparticles remain great challenges. In this study, genetically engineered polypeptide (PC(10)ARGD)-capped Te nanorods (P-TeNRs) was in situ synthesized. The excellent dispersion of P-TeNRs with average lengths of 25.2 +/- 4.65 nm and diameters of 6.5 +/- 0.83 nm were confirmed by TEM. PC(10)ARGD was integrated onto the surface of Te nanorods to enhance the stability and biocompatibility of P-TeNRs. P-TeNRs exhibited the excellent photothermal conversion performance under irradiation of NIR laser and the reactive oxygen species (ROS) generating capacity under ultrasound (US) irradiation, which can achieve photothermal therapy and sonodynamic therapy of tumors. Compared with single photothermal therapy and sonodynamic therapy, the results of in vitro and in vivo experiments showed that the combination therapy has a synergistic effect on breast 4T1 tumors. In addition, the results of in vivo toxicity assay suggested that synthesized P-TeNRs presented negligible toxicity. These results demonstrate that this P-TeNRs can be engineered as a novel theranostic nanoplatform for synergistic photothermal-sonodynamic therapy of tumors.