Atopic Patients Show Increased Interleukin 4 Plasma Levels but the Degree of Elevation Is Not Sufficient to Upregulate Interleukin-4-Sensitive Genes
Atopic Patients Show Increased Interleukin 4 Plasma Levels but the Degree of Elevation Is Not Sufficient to Upregulate Interleukin-4-Sensitive Genes
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DOI：
10.1159/000499431
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发表时间：
2019-05
影响因子：
2.7
通讯作者：
Eugenia Marbach-Breitrück;Andrea Kalledat;D. Heydeck;Sabine Stehling;J. Fluhr;T. Zuberbier;H. Kuhn
中科院分区：
文献类型：
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作者：
Eugenia Marbach-Breitrück;Andrea Kalledat;D. Heydeck;Sabine Stehling;J. Fluhr;T. Zuberbier;H. Kuhn
背景资料：特应性疾病构成了工业化国家的主要健康挑战，并且白细胞介素4（IL-4）水平升高常常表征这些疾病。以前的体外分析表明，IL-4强烈上调人单核细胞中IL-4敏感基因的表达。目的：为了探索类似的表达改变是否可能有助于体内特应性疾病的病理机制，我们进行了一项小规模的病例对照临床研究（n = 43），其中我们定量了IgE和IL-4的血浆水平以及血液白细胞中选定的IL-4敏感基因的表达。研究方法：34例过敏性鼻炎、过敏性湿疹和过敏性哮喘患者及9例健康对照者。通过ELISA测定IgE和IL-4血浆水平，并通过qRT-PCR定量血液白细胞中所选IL-4敏感基因产物的表达。此外，通过花生四烯酸氧合产物的RP-HPLC分析（离体活性测定）测量分离的单核细胞的脂肪酸氧合酶活性。结果如下：我们发现，过敏性患者的IgE和IL-4的血浆水平显著升高，但升高的程度不足以上调循环白细胞中选定的IL-4敏感基因的表达。此外，花生四烯酸加氧酶活性的血液单核细胞在特应性患者没有显着改变。结论：我们的数据表明，特应性患者的IL-4血浆水平不足以影响IL-4敏感基因的表达。
Background: Atopic diseases constitute a major health challenge for industrialized countries, and elevated levels of interleukin 4 (IL-4) frequently characterize these disorders. Previous in vitroanalyses have indicated that IL-4 strongly upregulates the expression of IL-4-sensitive genes in human monocytes. Objective: To explore whether similar expression alterations may contribute to the pathomechanisms of atopic diseases in vivo we carried out a small-scale case-control clinical study (n = 43), in which we quantified the plasma levels of IgE and IL-4 as well as the expression of selected IL-4-sensitive genes in blood leukocytes. Methods: 34 allergic patients suffering from allergic rhinitis (n = 11), atopic eczema (n = 11) and allergic asthma (n = 12) as well as 9 healthy control individuals were recruited. IgE and IL-4 plasma levels were determined by ELISA, and the expression of selected IL-4-sensitive gene products in blood leukocytes was quantified by qRT-PCR. In addition, the fatty acid oxygenase activity of isolated monocytes was measured by RP-HPLC analysis of the arachidonic acid oxygenation products (ex vivo activity assays). Results: We found that plasma levels of IgE and IL-4 were significantly elevated in atopic patients but the degree of elevation was not sufficient to upregulate the expression of the selected IL-4-sensitive genes in circulating leukocytes. Moreover, the arachidonic acid oxygenase activity of blood monocytes was not significantly altered in atopic patients. Conclusion: Our data suggest that the IL-4 plasma levels of atopic patients are not high enough to impact the expression of IL-4-sensitive genes.