The effects of changes in salinity on osmoregulation and chloride cell morphology of juvenile Australian snapper, Pagrus auratus
The effects of changes in salinity on osmoregulation and chloride cell morphology of juvenile Australian snapper, Pagrus auratus
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DOI：
10.1016/j.aquaculture.2007.08.043
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发表时间：
2007-11-26
期刊：
影响因子：
4.5
通讯作者：
Pankhurst, Patricia M.
中科院分区：
文献类型：
--
作者：
Fielder, D. Stewart;Allan, Geoff L.;Pankhurst, Patricia M.
幼澳大利亚笛鲷，Pagrus auratus从环境海水（30份/千）的快速转移到浓缩的高渗（45份/千）和稀释的高渗（15份/千）环境中的血清渗透压，血清[Na+]，[K+]，[Cl-]，血细胞比容和鳃氯细胞形态的影响进行了评估，在168小时后转移。血清渗透压、[Na+]、[K+]和[Cl-]在24小时后增加45份/千。与此相反，在15份/千24小时后，[K+]没有变化，但血清渗透压，[Na+]和[Cl-]下降。血清化学变化是短暂的，168 h后恢复到接近初始水平，分别为45 ppm和15 ppm。从千分之30转移到千分之45和千分之15不影响血液红细胞压积。采用Na+，K+-ATP酶特异性抗血清，通过免疫细胞化学染色技术，在所有盐度处理的笛鲷鳃丝和板层上皮中鉴定出鳃氯细胞。在45 ppm时，丝状和板层氯细胞的数量没有变化，但丝状氯细胞比板层氯细胞丰富。相比之下，丝状氯化物细胞的大小增加后72小时，并通过168小时后，从千分之30转移比初始大小的1.4倍。在15 ppm浓度下，72 h后，丝状氯细胞数量减少，丝状氯细胞和片状氯细胞的体积均减小。我们的研究结果表明，笛鲷可以在很宽的盐度范围内进行自我调节，并提供了间接的证据表明，丝状和片状氯细胞负责在高渗环境中从笛鲷排泄多余的盐。笛鲷在各种盐度下迅速适应和保持体内平衡的能力支持了这样一个事实，即笛鲷是池塘陆基水产养殖的合适物种，在池塘中盐度可能发生快速波动。皇冠版权所有（c）2007年出版的爱思唯尔B. V.保留所有权利。
The effect of rapid transfer of juvenile Australian snapper, Pagrus auratus from ambient seawater (30 parts per thousand) to concentrated hyperosmotic (45 parts per thousand) and diluted hyperosmotic (15 parts per thousand) environments on serum osmolality, serum [Na+], [K+], [Cl-], blood haematocrit and branchial chloride cell morphology was assessed during 168 h after transfer. Serum osmolality, [Na+], [K+] and [Cl-] increased after 24 h in 45 parts per thousand. In contrast, after 24 h in 15 parts per thousand, [K+] did not change but serum osmolality, [Na+] and [Cl-] decreased. The serum chemistry changes were transient and had returned to near initial levels after 168 h in 45 parts per thousand and 15 parts per thousand. Transfer from 30 parts per thousand to 45 parts per thousand and 15 parts per thousand did not affect blood haematocrit. Branchial chloride cells were identified in both filament and lamellar epithelia of snapper held in all salinity treatments by an immunocytochemical staining technique using an antiserum specific for Na+, K+-ATPase. In 45 parts per thousand, the number of filament and lamellar chloride cells did not change, but filament chloride cells were more abundant than lamellar chloride cells. In contrast, filament chloride cells had increased in size after 72 h and by 168 h after transfer from 30 parts per thousand were 1.4-fold larger than the initial size. In 15 parts per thousand, the number of filament chloride cells and the size of both filament and lamellar chloride cells had decreased after 72 h. Our results demonstrate that snapper can osmoregulate in a wide range of salinity and provide indirect evidence that both filament and lamellar chloride cells are responsible for excretion of excess salt from snapper in hyperosmotic environments. The ability for snapper to adapt rapidly and maintain homeostasis in a wide range of salinities supports the fact that snapper are a suitable species for land-based aquaculture in ponds, where rapid fluctuation in salinity can occur. Crown Copyright (c) 2007 Published by Elsevier B.V. All rights reserved.