Simple method for reduction of autofluorescence in fluorescence microscopy
Simple method for reduction of autofluorescence in fluorescence microscopy
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DOI：
10.1177/002215540205000315
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发表时间：
2002-03-01
影响因子：
3.2
通讯作者：
Gabel, D
中科院分区：
文献类型：
--
作者：
Neumann, M;Gabel, D
固定神经组织的自体荧光常常使荧光显微镜的使用复杂化。背景荧光可以通过在用荧光探针处理之前用光照射而显著减少或消除，从而导致更高的对比度而不会不利地影响染色概率。
Autofluorescence of aldehyde-fixed neural tissue often complicates the use of fluorescence microscopy. Background fluorescence can be notably reduced or eliminated by irradiation with light before treatment with fluorescence probes, resulting in a higher contrast without adversely affecting the staining probabilities.