Expression patterns of the novel imprinted genes Nap1l5 and Peg13 and their non-imprinted host genes in the adult mouse brain
Expression patterns of the novel imprinted genes Nap1l5 and Peg13 and their non-imprinted host genes in the adult mouse brain
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DOI：
10.1016/j.modgep.2004.03.008
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发表时间：
2004-10-01
影响因子：
1.2
通讯作者：
Wilkinson, LS
中科院分区：
文献类型：
--
作者：
Davies, W;Smith, RJ;Wilkinson, LS
最近的工作表明印记基因在神经发育和行为中发挥作用，并且定义这些基因在脑组织中的表达模式已成为建立功能的关键先决条件。在这项工作中，我们使用原位杂交方法报告了成年小鼠大脑中两个新的印记基因座 Napl15 和 Peg13 的表达模式。 Napl15 和 Peg13 分别位于小鼠 6 号和 15 号染色体上两个独立微印迹结构域中的非印迹基因 Herc3 和 Tularik1 (T1)/KIAA1882 同源物的内含子内。这些“宿主”基因在大脑中高度表达，因此我们有兴趣评估它们与印迹基因平行的表达模式。所有四种基因的大脑表达似乎主要是神经元的。 Napl15和Peg13的详细表达谱总体相似，表达广泛，在间隔区和下丘脑区、海马区和大脑皮层表达相对较高。相比之下，印记基因与其非印记宿主之间存在一定程度的分离，因为虽然Herc3和T1/KIAA1882同源物再次广泛表达，但这些基因在浦肯野神经元和梨状皮层中也特别高表达。我们还检查了肾上腺中新型印记基因的表达。 Napl15 表达主要位于肾上腺髓质，而 Peg13 表达更广泛地观察到整个肾上腺髓质和外皮质层。 (C) 2004 Elsevier B.V. 保留所有权利。
Recent work has implicated imprinted gene functioning in neurodevelopment and behaviour and defining the expression patterns of these genes in brain tissue has become a key prerequisite to establishing function. In this work we report on the expression patterns of two novel imprinted loci, Napl15 and Peg13, in adult mouse brain using in situ hybridisation methods. Napl15 and Peg13 are located, respectively, within the introns of the non-imprinted genes Herc3 and the Tularik1 (T1)/KIAA1882 homologue in two separate microimprinted domains on mouse chromosomes 6 and 15. These 'host' genes are highly expressed in brain and consequently we were interested in assessing their expression patterns in parallel to the imprinted genes. The brain expression of all four genes appeared to be mainly neuronal. The detailed expression profiles of Napl15 and Peg13 were generally similar with widespread expression that was relatively high in the septal and hypothalamic regions, the hippocampus and the cerebral cortex. In contrast, there was some degree of dissociation between the imprinted genes and their non-imprinted hosts, in that, whilst there was again widespread expression of Herc3 and the T1/KIAA1882 homologue, these genes were also particularly highly expressed in Purkinje neurons and piriform, cortex. We also examined expression of the novel imprinted genes in the adrenal glands. Napl15 expression was localised mainly to the adrenal medulla, whilst Peg13 expression was observed more generally throughout the adrenal medulla and the outer cortical layers. (C) 2004 Elsevier B.V. All rights reserved.