Identification and characterization of Lisianthus necrotic ringspot virus, a novel distinct tospovirus species causing necrotic disease of lisianthus (Eustoma grandiflorum)
Identification and characterization of Lisianthus necrotic ringspot virus, a novel distinct tospovirus species causing necrotic disease of lisianthus (Eustoma grandiflorum)
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DOI：
10.1007/s10327-014-0503-9
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发表时间：
2014-01
影响因子：
1.2
通讯作者：
Y. Shimomoto;K. Kobayashi;M. Okuda
中科院分区：
文献类型：
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作者：
Y. Shimomoto;K. Kobayashi;M. Okuda
2007年，在日本高知县发现了叶子上有坏死环斑的洋桔梗（洋桔梗）植物。番茄斑萎病毒样球形囊膜颗粒。电镜下观察到直径约160 nm的细胞。测定了该病毒的S RNA片段的完整核苷酸序列，并利用核衣壳蛋白和非结构S蛋白的推导氨基酸序列进行了系统发育分析，表明该病毒在遗传学上与任何已知的番茄斑萎病毒属物种不同。结果表明，该病毒为布尼亚病毒科番茄斑病毒属的新成员。该病毒是日本洋桔梗中自然发生的第五种不同的番茄斑萎病毒。机械接种后，洋桔梗幼苗出现坏死症状。还研究了病毒分离物在几种试验植物上的寄主范围。
In 2007, lisianthus (Eustoma grandiflorum) plants with necrotic ringspots on the leaves were found in Kochi Prefecture, Japan. Tospovirus-like spherical enveloped particles with ca. 160 nm in diameter were observed with electron microscopy. The complete nucleotide sequence of the S RNA segment of the virus was determined, and phylogenetic analysis using deduced amino acid sequences of the nucleocapsid protein and the nonstructural S protein indicated that the virus is phylogenetically distinct from any known tospovirus species. The results suggest that the virus is a new member of the genusTospovirus, in the familyBunyaviridae. The virus is the fifth distinct tospovirus occurring naturally in lisianthus in Japan. The necrotic symptoms were reproduced on lisianthus seedlings after mechanical inoculation. The host range of the virus isolate on several test plants was also examined.